Functionalized polystyrene supports for solid-phase synthesis of glycyl-, alanyl-, and isoleucyl-RNA conjugates as hydrolysis-resistant mimics of peptidyl-tRNAs
| Autor: | Jessica Steger, Ronald Micura |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Adenosine
Solid-phase synthesis Stereochemistry Clinical Biochemistry Glycine Oligonucleotides Pharmaceutical Science RNA Transfer Amino Acyl 010402 general chemistry 01 natural sciences Biochemistry Ribosome Article Hydrolysis chemistry.chemical_compound Amide Azido nucleoside Drug Discovery Isoleucine tRNA Molecular Biology Solid-Phase Synthesis Techniques ComputingMethodologies_COMPUTERGRAPHICS Medicine(all) Alanine 010405 organic chemistry Chemistry Oligonucleotide Organic Chemistry RNA Ribosomal RNA 0104 chemical sciences 3. Good health enzymes and coenzymes (carbohydrates) Transfer RNA Polystyrenes Molecular Medicine RNA-peptide conjugates Peptides Ribosomes |
| Zdroj: | Bioorganic & Medicinal Chemistry |
| ISSN: | 0968-0896 |
| DOI: | 10.1016/j.bmc.2011.07.018 |
| Popis: | Graphical abstract RNA-peptide conjugates that mimic amino acid-charged tRNAs and peptidyl-tRNAs are of high importance for structural and functional investigations of ribosomal complexes. Here, we present the synthesis of glycyl-, alanyl-, and isoleucyladenosine modified solid supports that are eligible for the synthesis of stable 3′-aminoacyl- and 3′-peptidyl-tRNA termini with an amide instead of the natural ester linkage. The present work significantly expands the range of accessible peptidyl-tRNA mimics for ribosomal studies. |
| Databáze: | OpenAIRE |
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