Characterization of New Zealand White Rabbit Gut-Associated Lymphoid Tissues and Use as Viral Oncology Animal Model
| Autor: | Michael Dale Lairmore, Stefan Niewiesk, Robyn Haines, Rebeccah A. Urbiztondo, Rashade A H Haynes, Elaine M. Simpson |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
CD4-Positive T-Lymphocytes
0301 basic medicine Pathology medicine.medical_specialty Lymphoid Tissue T cell CD8-Positive T-Lymphocytes Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Intestine Small medicine Animals Mesenteric lymph nodes Cytotoxic T cell Lymph node B cell Lamina propria Articles General Medicine Disease Models Animal 030104 developmental biology medicine.anatomical_structure Lymphatic system 030220 oncology & carcinogenesis Immunology Intraepithelial lymphocyte Animal Science and Zoology Rabbits |
| Zdroj: | ILAR Journal. 57:34-43 |
| ISSN: | 1930-6180 1084-2020 |
| DOI: | 10.1093/ilar/ilw004 |
| Popis: | Rabbits have served as a valuable animal model for the pathogenesis of various human diseases, including those related to agents that gain entry through the gastrointestinal tract such as human T cell leukemia virus type 1. However, limited information is available regarding the spatial distribution and phenotypic characterization of major rabbit leukocyte populations in mucosa-associated lymphoid tissues. Herein, we describe the spatial distribution and phenotypic characterization of leukocytes from gut-associated lymphoid tissues (GALT) from 12-week-old New Zealand White rabbits. Our data indicate that rabbits have similar distribution of leukocyte subsets as humans, both in the GALT inductive and effector sites and in mesenteric lymph nodes, spleen, and peripheral blood. GALT inductive sites, including appendix, cecal tonsil, Peyer's patches, and ileocecal plaque, had variable B cell/T cell ratios (ranging from 4.0 to 0.8) with a predominance of CD4 T cells within the T cell population in all four tissues. Intraepithelial and lamina propria compartments contained mostly T cells, with CD4 T cells predominating in the lamina propria compartment and CD8 T cells predominating in the intraepithelial compartment. Mesenteric lymph node, peripheral blood, and splenic samples contained approximately equal percentages of B cells and T cells, with a high proportion of CD4 T cells compared with CD8 T cells. Collectively, our data indicate that New Zealand White rabbits are comparable with humans throughout their GALT and support future studies that use the rabbit model to study human gut-associated disease or infectious agents that gain entry by the oral route. |
| Databáze: | OpenAIRE |
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