Abscisic Acid and Gibberellin Differentially Regulate Expression of Genes of the SNF1-Related Kinase Complex in Tomato Seeds
| Autor: | Hong Yang, A. Bruce Downie, Kent J. Bradford, Peetambar Dahal, Oliver H. Gee, Veria Y. Alvarado |
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| Rok vydání: | 2003 |
| Předmět: |
Physiology
Protein subunit Molecular Sequence Data Saccharomyces cerevisiae Germination Plant Science Protein Serine-Threonine Kinases chemistry.chemical_compound Solanum lycopersicum Gene Expression Regulation Plant Two-Hybrid System Techniques Genetics Amino Acid Sequence RNA Messenger Protein kinase A Abscisic acid Cellular localization biology Genetic Complementation Test fungi Gene Expression Regulation Developmental food and beverages biology.organism_classification Gibberellins chemistry Biochemistry Multigene Family Seeds Dormancy Gibberellin Abscisic Acid Protein Binding Research Article |
| Zdroj: | Plant Physiology. 132:1560-1576 |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.102.019141 |
| Popis: | The SNF1/AMP-activated protein kinase subfamily plays central roles in metabolic and transcriptional responses to nutritional or environmental stresses. In yeast (Saccharomyces cerevisiae) and mammals, activating and anchoring subunits associate with and regulate the activity, substrate specificity, and cellular localization of the kinase subunit in response to changing nutrient sources or energy demands, and homologous SNF1-related kinase (SnRK1) proteins are present in plants. We isolated cDNAs corresponding to the kinase (LeSNF1), regulatory (LeSNF4), and localization (LeSIP1 and LeGAL83) subunits of the SnRK1 complex from tomato (Lycopersicon esculentum Mill.). LeSNF1 and LeSNF4 complemented yeast snf1 and snf4 mutants and physically interacted with each other and with LeSIP1 in a glucose-dependent manner in yeast two-hybrid assays. LeSNF4 mRNA became abundant at maximum dry weight accumulation during seed development and remained high when radicle protrusion was blocked by abscisic acid (ABA), water stress, far-red light, or dormancy, but was low or undetected in seeds that had completed germination or in gibberellin (GA)-deficient seeds stimulated to germinate by GA. In leaves, LeSNF4 was induced in response to ABA or dehydration. In contrast, LeSNF1 and LeGAL83 genes were essentially constitutively expressed in both seeds and leaves regardless of the developmental, hormonal, or environmental conditions. Regulation of LeSNF4 expression by ABA and GA provides a potential link between hormonal and sugar-sensing pathways controlling seed development, dormancy, and germination. |
| Databáze: | OpenAIRE |
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