Isolation of recombinant cDNAs encoding chicken erythroid delta-aminolevulinate synthase
| Autor: | Norio Hayashi, Nelson S. Yew, James Douglas Engel, Jerry B. Dodgson, Mark Federspiel, Masayuki Yamamoto |
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| Rok vydání: | 1985 |
| Předmět: |
Erythrocytes
Multidisciplinary Expression vector cDNA library DNA Recombinant DNA Molecular cloning Biology Isozyme Molecular biology Gene Expression Regulation Liver Biochemistry Transcription (biology) Complementary DNA Gene expression Animals RNA Messenger Chickens Gene Immunosorbent Techniques 5-Aminolevulinate Synthetase Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 82:3702-3706 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.82.11.3702 |
| Popis: | We report the isolation of cDNA clones encoding delta-aminolevulinate synthase (ALA synthase; EC 2.3.1.37), the first enzyme in the heme biosynthetic pathway in animal cells. The gene was isolated from a chicken erythroid cDNA library prepared in the bacteriophage lambda fusion/expression vector gt11, using rabbit antibody raised against the relatively abundant chicken liver enzyme. The chicken liver and red cell ALA synthase isozymes share substantial crossreactivity to the antibody, thereby allowing isolation of the erythroid-specific gene by using the heterologous antibody in immune screening of the red cell cDNA library. Preliminary analysis documenting the tissue specificity of transcription indicates that the enzyme is encoded by a highly homologous set of messages, which appear to differ in size in various avian tissues. From analysis using strand-specific RNA probes, it appears that the different ALA synthase mRNAs detected may be transcribed from a family of genes that are closely related in nucleotide sequence and are each regulated in a developmentally specific manner. |
| Databáze: | OpenAIRE |
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