Reverse transcription recombinase-aided amplification assay for rapid detection of the influenza A(H1N1)pdm09 H275Y mutation that confers oseltamivir resistance
| Autor: | Gang Zhao, Ren-hua Chen, Shu-chang Chen, Guo-zhong Zhang, Xin-fen Yu, Jun Li, Yang Zi, Xu-hui Yang, Hao-qiu Wang, Lingfeng Mao |
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| Rok vydání: | 2021 |
| Předmět: |
Oseltamivir
medicine.drug_class Mutation Missense Neuraminidase Biology Virus Recombinases chemistry.chemical_compound Influenza A Virus H1N1 Subtype Degree Celsius Drug Resistance Viral Influenza Human medicine Recombinase Humans Molecular Biology Neuraminidase inhibitor Reverse Transcription Cell Biology Resistance mutation Virology Reverse transcriptase chemistry Mutation Mutation (genetic algorithm) |
| Zdroj: | Molecular and Cellular Probes. 60:101771 |
| ISSN: | 0890-8508 |
| Popis: | The emergence of the influenza A(H1N1)pdm09 virus with the NA-H275Y mutation, which confers oseltamivir resistance, must be monitored, especially in patients undergoing neuraminidase inhibitor treatment. In this study, we developed a reverse transcription recombinase-aided amplification assay that has high sensitivity (detection limit: 1.0 × 101 copies/μL) and specificity for detecting the oseltamivir-resistant H275Y mutation; the assay is performed within 30 min at a constant temperature of 39° Celsius using an isothermal device. This method is suitable for the clinical application of targeted testing, thereby providing technical support for precision medicine in individual drug applications for patients with severe infection or immunosuppression. |
| Databáze: | OpenAIRE |
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