A rapid change in microbial communities of the shale gas drilling fluid from 3548 m depth to the above-ground storage tank
| Autor: | Hong Mei Mu, Bai Chun Wu, Yang Li, Hailiang Dong, Yun Yang Wan, Tian Yan, Cheng Gang Xian |
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| Rok vydání: | 2020 |
| Předmět: |
China
Environmental Engineering 010504 meteorology & atmospheric sciences Firmicutes Soil science 010501 environmental sciences Natural Gas Wastewater 01 natural sciences chemistry.chemical_compound Drilling fluid RNA Ribosomal 16S Environmental Chemistry Humans Oil and Gas Fields Waste Management and Disposal 0105 earth and related environmental sciences geography geography.geographical_feature_category biology Microbiota Sediment Drilling biology.organism_classification Inlet Pollution chemistry Microbial population biology Storage tank Petroleum Environmental science |
| Zdroj: | The Science of the total environment. 784 |
| ISSN: | 1879-1026 |
| Popis: | Despite the growing body of studies on the various fracturing phrases, the research on the differences between subterranean and surface microorganisms at shale gas drilling sites is still limited. Generally, shale gas development and the production process are divided into drilling and fracturing. The distribution of microbial communities in the latter has been paid some attention, but a deficit remains in terms of our understanding of the microbial community in the former, especially for the phase of drilling flowback and drilling flowback surface. In this study, four drilling flowback fluids (DFFs) (H230-flowback drilling cuttings, H23G-flowback drilling mud, H240-flowback drilling sediment, and H21F-flowback drilling water) from the outlet of subterranean pipeline to the inlet of storage tank were successively collected from H2 shale gas field during its initial drilling in Sichuan, China. Natural mountain water (H10W) used as the injection water of H2 was also sampled. Illumina MiSeq 16S rRNA gene sequencing revealed a total of 8 phyla, 17 classes, 36 orders, 62 families, and 98 genera that were recovered from these samples with uneven distribution. The majority of the obtained sequences belonged to the phyla Proteobacteria (75.36%), Bacteroidetes (10.75%), and Firmicutes (5.64%), with significant differences found in DFFs and injection water. The richness of microorganisms gradually increased with the increasing flowback flowing distance (H230 |
| Databáze: | OpenAIRE |
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