Duplicate insulin-like growth factor-I genes in salmon display alternative splicing pathways
| Autor: | Anne E. Wallis, R H Devlin |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Transcription
Genetic medicine.medical_treatment Molecular Sequence Data Polymerase Chain Reaction Homology (biology) Endocrinology Salmon Sequence Homology Nucleic Acid biology.animal Complementary DNA medicine Animals Amino Acid Sequence Cloning Molecular Insulin-Like Growth Factor I Molecular Biology Gene Genetics Base Composition Base Sequence Sequence Homology Amino Acid biology Growth factor Alternative splicing Nucleic acid sequence Vertebrate DNA General Medicine Blotting Northern Alternative Splicing Blotting Southern Multigene Family RNA splicing RNA Sequence Alignment |
| Zdroj: | Molecular Endocrinology. 7:409-422 |
| ISSN: | 1944-9917 0888-8809 |
| Popis: | Insulin-like growth factor (IGF-I) is a single chain, 70-amino acid, peptide hormone, which is essential to the process of growth and differentiation in higher vertebrates. IGF-I exhibits a very high degree of conservation throughout vertebrate evolution, and recent studies in fish have shown improved rates of growth in salmonids treated with exogenous recombinant mammalian IGF-I, implying a role for this hormone in fish similar to that in mammals. This paper reports the identification and sequencing of four different liver-derived cDNAs encoding IGF-I from chinook salmon, Oncorhynchus tshawytscha. The different cDNAs all contain sequences that encode an identical mature IGF-I protein. The differences between the four cDNAs are of two types: 1) single basepair changes and 2) additional blocks of sequence occurring at one site within the E-domain regions of three of the four cDNAs. The sequence data indicate that each of the cDNAs identified was transcribed from a different IGF-I gene, and analysis of the chinook salmon genome by Southern blotting supports the existence of four distinct IGF-I genes and suggests that there are at least two IGF-I loci in salmon. Although, analysis of total RNA from salmonid liver identifies only a single major transcript of 4200 nucleotides, polymerase chain reaction analysis suggests the presence of two structurally different salmonid IGF-I genes with the potential for multiple splicing alternatives of the IGF-I transcript. |
| Databáze: | OpenAIRE |
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