Mode of action studies on the formation of enamel minerals from a novel toothpaste containing calcium silicate and sodium phosphate salts

Autor: Xiping Feng, Jianing N. Sun, Renjiang Liu, Weining Liu, Jinfang Wang, Yuekui Sun, Mel Carvell, Danying Tao, Andrew Joiner, Hui Chen, Qinghong Hu, Yan Deng, Xiaoke Li
Rok vydání: 2014
Předmět:
Calcium Phosphates
Toothbrushing
Time Factors
business.product_category
Materials science
Scanning electron microscope
Sodium
chemistry.chemical_element
Dentistry
In Vitro Techniques
Phosphates
Fluorides
Random Allocation
chemistry.chemical_compound
Microscopy
Electron
Transmission
stomatognathic system
Materials Testing
Spectroscopy
Fourier Transform Infrared
Animals
Humans
Dental Pellicle
Fourier transform infrared spectroscopy
Dental Enamel
General Dentistry
Crystallography
Toothpaste
Enamel paint
business.industry
Silicates
Saliva
Artificial
Spectrometry
X-Ray Emission
Calcium Compounds
stomatognathic diseases
Durapatite
chemistry
visual_art
Calcium silicate
Microscopy
Electron
Scanning
visual_art.visual_art_medium
Calcium
Cattle
Selected area diffraction
business
Fluoride
Toothpastes
Follow-Up Studies
Nuclear chemistry
Zdroj: Journal of Dentistry. 42:S30-S38
ISSN: 0300-5712
DOI: 10.1016/s0300-5712(14)50005-x
Popis: Objectives To investigate in vitro and in situ the deposition and formation of hydroxyapatite (HAP) on enamel surfaces following brushing with a novel toothpaste containing calcium silicate (CaSi), sodium phosphate salts and fluoride. Methods Polished enamel blocks were brushed in vitro with a slurry of the CaSi toothpaste. After one brush and four weeks simulated brushing the enamel surfaces were analysed. In an in situ protocol, enamel blocks were attached to first or second molar teeth of healthy subjects, exposed to 4 weeks twice per day brushing with the CaSi toothpaste and then analysed. The surface deposits were analysed using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM) and selected area electron diffraction (SAED). In addition, the CaSi toothpaste was slurried in simulated oral fluid (SOF) over a 3 hour period and the solids were isolated and analysed by Fourier transform infrared spectroscopy (FTIR). Results The FTIR study demonstrated that calcium phosphate phases had formed and these became increasingly crystalline over 3 hours. CaSi was deposited onto enamel surfaces following one brushing with the toothpaste in vitro.The deposited particles showed evidence of HAP crystalline phases associated with the CaSi. Following 4 weeks brushing in vitro, the deposition increased and analyses showed that the deposited material was HAP. These results were confirmed by the in situ study. Conclusions Calcium silicate can be deposited onto enamel surfaces from a novel toothpaste formulation where it can form the enamel mineral HAP. Clinical Significance A novel toothpaste formulation containing CaSi can form HAP on enamel surfaces. The potential of this technology is for a novel approach to the repair of demineralised enamel and the protection of enamel during acid exposure.
Databáze: OpenAIRE
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