Generation of highly differentiated BHY oral squamous cell carcinoma multicellular spheroids
| Autor: | Denise Sievers, Henning Schliephake, Jens Bunzendahl, Christina Perske, Phillipp Brockmeyer, Alice Frosch, Bernhard Hemmerlein |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Oncogene Chemistry Cell Spheroid Articles Cell cycle Molecular medicine 03 medical and health sciences 3D cell culture stomatognathic diseases 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Oncology Cell culture Apoptosis 030220 oncology & carcinogenesis Cancer research medicine |
| Popis: | Three-dimensional (3D) multicellular spheroids (MCS) are considered suitable models in cancer research and anticancer drug development. Although studying the complex tumour characteristics from all different degrees of malignancy is vital, MCS generation has only been described in a few moderately- and poorly differentiated oral squamous cell carcinoma (OSCC) cell lines. No previous study has demonstrated the MCS formation in a highly differentiated OSCC cell line. For the first time, the present study aimed to generate MCS from the highly differentiated OSCC cell line BHY. BHY spheroids were grown in three independent experiments in 96-well plates through the use of the liquid overlay technique. Although BHY cells are grow slowly and are difficult to culture, they formed compact MCS within 24 h. After 3 days of incubation, no further increase in spheroid size was observed. MCS were harvested, paraffin-embedded and 2 µm tissue sections were prepared for further analysis. The diameter and volume of each spheroid were determined. BHY MCS diameter ranged between 46.76 and 233.26 µm, with a volume range from 5.35×104-6.65×106 µm3. In conclusion, using the liquid overlay technique, the highly differentiated OSCC cell line BHY forms different sized spheroids, which may be used for further investigations. |
| Databáze: | OpenAIRE |
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