Kinetics of erythrocyte swelling and membrane hole formation in hypotonic media
| Autor: | A. Pribush, Dan Meyerstein, Naomi Meyerstein |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Lysis
Erythrocytes Time Factors Osmotic shock Cytochalasin B Analytical chemistry Biophysics Aquaporins Electric Capacitance Hemolysis Biochemistry Hypotonic medium chemistry.chemical_compound Capacitance/conductance medicine Humans Cell Size Water transport Chemistry Osmolar Concentration Electric Conductivity Conductance Water Cell Biology Elasticity Culture Media Kinetics Red blood cell Membrane Hypotonic Solutions Glutaral Tonicity Swelling medicine.symptom |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. (2):119-132 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/S0005-2736(01)00418-7 |
| Popis: | Red blood cell (RBC) swelling and membrane hole formation in hypotonic external media were studied by measuring the time-dependent capacitance, C, and the conductance, G, in the beginning of the beta-dispersion range. At high and moderate osmolarities of the external solution the capacitance reaches a steady-state whereas at low osmolarities it reveals a biphasic kinetics. Examination of RBC suspensions exposed to different concentrations of HgCl(2) demonstrates that water transport through mercury-sensitive water channel controls RBC swelling. Unlike the capacitance, an increase in the conductance to a stationary level is observed after a certain delay. A comparison of G(t) curves recorded for the suspensions of the intact cells and those treated with cytochalasin B or glutaraldehyde demonstrates the significant effect of the membrane viscoelasticity on the pore formation. It is shown that the stretched membrane of completely swollen RBC retains its integrity for a certain time, termed as the membrane lifetime, t(memb). Therefore, the resistivity of RBCs to a certain osmotic shock may be quantified by the distribution function of RBC(t(memb)). |
| Databáze: | OpenAIRE |
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