Paeoniflorin inhibits excitatory amino acid agonist-and high-dose morphine-induced nociceptive behavior in mice via modulation of N-methyl-D-aspartate receptors
| Autor: | Huei-Yann Tsai, Jhao-Guei Yang, Yu-Chien Chen, Hsun-Lang Fang, Yuh-Fung Chen, Ming-Ming Lee |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Paeonia lactiflora Excitatory amino acid agonists Paeoniflorin Pharmacology Receptors N-Methyl-D-Aspartate Oligodeoxyribonucleotides Antisense Mice 03 medical and health sciences Glutamatergic chemistry.chemical_compound 0302 clinical medicine Glucosides Animals Medicine Excitatory Amino Acid Agonist Analgesics Mice Inbred ICR Behavior Animal Morphine biology business.industry Anti-Inflammatory Agents Non-Steroidal General Medicine NR2B NMDA receptor biology.organism_classification Nociceptive behavior 030104 developmental biology Nociception nervous system Complementary and alternative medicine chemistry Antisense oligodeoxynucleotides High-dose morphine Monoterpenes Excitatory postsynaptic potential NBQX business 030217 neurology & neurosurgery Research Article |
| Zdroj: | BMC Complementary and Alternative Medicine |
| ISSN: | 1472-6882 |
| DOI: | 10.1186/s12906-016-1230-x |
| Popis: | Background Pain, the most common reasons for physician consultation, is a major symptom in many medical conditions that can significantly interfere with a person’s life quality and general functioning. Almost all painkillers have its untoward effects. Therefore, seeking for a safe medication for pain relieve is notable nowadays. Paeonia lactiflora is a well-known traditional Chinese medicine. Paeoniflorin is an active component found in Paeonia lactiflora, which has been reported to inhibit formalin-induced nociceptive behavior in mice. Aims of this present study were to investigate effects of paeoniflorin on excitatory amino acid agonist- or high-dose morphine-induced nociceptive behaviors in mice. Results Paeoniflorin (100, 200, 500 nmol, i.c.v.) alone and combined with glutamatergic antagonists (MK-801 14.8 pmol, or NBQX 5 nmol, i.t.) inhibited nociception. Those agents also inhibited the clonic seizure-like excitation induced by high-dose morphine (250 nmol, i.t) in mice. Antisense oligodeoxynucleotides of NMDA receptor subunits NR1, NR2A, NR2B significantly enhanced the inhibition of paeoniflorin on excitatory amino acid-and high-dose morphine-induced nociception. Docking energy data revealed that paeoniflorin had stronger binding activity in NR2A and NR2B than NR2C of NMDA receptors. Conclusions Results of this study indicate that paeoniflorin-induced inhibition of excitatory amino acid agonist- and high-dose morphine-induced nociceptive behaviors might be due to modulation of NMDA receptors, specifically the NR2B subunit. |
| Databáze: | OpenAIRE |
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