HLA Class II-Like Antiidiotypic Antibodies from Highly Sensitized Patients Inhibit T-Cell Alloresponses
| Autor: | N. DenHollander, Nashrudeen Hack, T. McKnight, Carl J. Cardella, Sarita Angra |
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| Rok vydání: | 2008 |
| Předmět: |
Male
T-Lymphocytes T cell Human leukocyte antigen Lymphocyte Activation Major histocompatibility complex Immune system Immunoglobulin Idiotypes HLA Antigens medicine Humans Immunology and Allergy Pharmacology (medical) Cells Cultured B-Lymphocytes Transplantation biology business.industry Immune Sera T-cell receptor Histocompatibility Antigens Class II Panel reactive antibody T lymphocyte Antibodies Anti-Idiotypic medicine.anatomical_structure Immunoglobulin G Immunology biology.protein Female Immunization Antibody business |
| Zdroj: | American Journal of Transplantation. 8:111-120 |
| ISSN: | 1600-6135 |
| DOI: | 10.1111/j.1600-6143.2007.02043.x |
| Popis: | The purpose of this study is to identify factors in the sera of highly sensitized (HS) patients (pts) that inhibit T-cell alloresponses. An in vitro assay was used to measure HLA class I and class II-like antiidiotypic antibodies (anti-ids). The stimulation index (SI) was used to measure PBL and T-cell responses to alloantigens. All HS sera (32 pts) and the IgG fraction inhibited PBL and CD4(+) T-cell responses to alloantigens. The SI with HS IgG was 7.9 +/- 1.7 as compared to 31.5 +/- 5.9 with normal IgG (p = 0.0003). In a subset of pts who were transiently sensitized, the SI was 6.6 +/- 1.0 with a high panel reactive antibody (PRA), but when their PRA was zero, the SI was 17.8 +/- 1.3 (p = 0.0000001). Anti-ids were found in 100% of 17 pts with a high PRA. The T-cell inhibitory factors reduced CD4(+) T-cell responses of HS pts to alloantigens in the presence of autologous anti-ids, were MHC restricted and were inactivated by in vitro generated antibodies to HLA class II-like anti-ids. The HLA class II-like anti-id IgG molecules bind to the TCR of CD4(+) T cells and may impair their ability to help in the downregulating antibody response to anti-ids. |
| Databáze: | OpenAIRE |
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