Highly flexible metabolism of the marine euglenozoan protist Diplonema papillatum
| Autor: | Kristína Záhonová, Peter Barath, Anton Horváth, Ingrid Škodová-Sveráková, Veronika Lukáčová, Anzhelika Butenko, Lenka Kohútová, Julius Lukeš, Aleš Horák, Martin Moos, Galina Prokopchuk, Barbora Bučková, Maksym Danchenko, Valéria Juricová, Petr Šimek, Drahomíra Faktorová |
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| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Diplonema Bioenergetics Physiology QH301-705.5 Euglenozoa Plant Science Biology Pentose phosphate pathway medicine.disease_cause General Biochemistry Genetics and Molecular Biology Metabolomics Structural Biology medicine Glycolysis Biology (General) Meiotic Prophase I Mitochondrion Adaptation Hypoxia Ecology Evolution Behavior and Systematics Phylogeny Protist Eukaryota Cell Biology Metabolism biology.organism_classification Multiomics Citric acid cycle Oxygen Biochemistry General Agricultural and Biological Sciences Developmental Biology Biotechnology Research Article |
| Zdroj: | BMC Biology BMC Biology, Vol 19, Iss 1, Pp 1-21 (2021) |
| ISSN: | 1741-7007 |
| Popis: | Background The phylum Euglenozoa is a group of flagellated protists comprising the diplonemids, euglenids, symbiontids, and kinetoplastids. The diplonemids are highly abundant and speciose, and recent tools have rendered the best studied representative, Diplonema papillatum, genetically tractable. However, despite the high diversity of diplonemids, their lifestyles, ecological functions, and even primary energy source are mostly unknown. Results We designed a metabolic map of D. papillatum cellular bioenergetic pathways based on the alterations of transcriptomic, proteomic, and metabolomic profiles obtained from cells grown under different conditions. Comparative analysis in the nutrient-rich and nutrient-poor media, as well as the absence and presence of oxygen, revealed its capacity for extensive metabolic reprogramming that occurs predominantly on the proteomic rather than the transcriptomic level. D. papillatum is equipped with fundamental metabolic routes such as glycolysis, gluconeogenesis, TCA cycle, pentose phosphate pathway, respiratory complexes, β-oxidation, and synthesis of fatty acids. Gluconeogenesis is uniquely dominant over glycolysis under all surveyed conditions, while the TCA cycle represents an eclectic combination of standard and unusual enzymes. Conclusions The identification of conventional anaerobic enzymes reflects the ability of this protist to survive in low-oxygen environments. Furthermore, its metabolism quickly reacts to restricted carbon availability, suggesting a high metabolic flexibility of diplonemids, which is further reflected in cell morphology and motility, correlating well with their extreme ecological valence. |
| Databáze: | OpenAIRE |
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