Kinetics of uptake and subcellular distribution of human growth hormone in liver slices
| Autor: | V.T. Maddaiah, Platon J. Collipp, S. Chen, I. Rezvani, J. Thomas |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
medicine.medical_specialty
Kinetics Mitochondria Liver Biology Tritium Growth hormone Binding Competitive Biochemistry Cytosol Species Specificity Internal medicine medicine Animals Humans Bovine somatotropin Incubation Hypophysectomy Cell Nucleus Nitrates Human growth hormone Uptake kinetics Rats Subcellular distribution Endocrinology Liver Growth Hormone Pituitary Gland Microsomes Liver Cattle Hormone |
| Zdroj: | Biochemical Medicine. 7:432-440 |
| ISSN: | 0006-2944 |
| DOI: | 10.1016/0006-2944(73)90064-1 |
| Popis: | Uptake of 3 H-acetyl human growth hormone ( 3 H-HGH) and 3 H-acetyl bovine growth hormone ( 3 H-BGH) into slices of hypophysectomized rat liver and human liver (obtained from autopsy) has been measured both as a function of time and concentration of the labeled hormones in the incubation medium. Uptake kinetics as a function of concentration of 3 H-HGH or 3 H-BGH in rat liver slices followed a sigmoid curve. Similar sigmoid curve was obtained when increasing concentrations of 3 H-HGH were incubated with human liver slices. In contrast, uptake kinetics of 3 H-BGH in human liver slices followed a hyperbolic curve. Addition of unlabeled HGH or BGH into the incubation medium reduced the uptake of 3 H-HGH in rat liver slices but in human liver slices only human growth hormone competed. This binding-site difference along with the reported molecular differences between human and bovine growth hormones may well explain the different biological specificities of the two hormones. |
| Databáze: | OpenAIRE |
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