Toll-Like Receptor 4 Signaling in the Ileum and Colon of Gnotobiotic Piglets Infected with Salmonella Typhimurium or Its Isogenic ∆rfa Mutants
| Autor: | Alla Splichalova, Daniela Karasova, Iva Splichalova, Ivan Rychlik, Igor Splichal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Salmonella
endotoxin Lipopolysaccharide Health Toxicology and Mutagenesis toll-like receptor 4 gnotobiotic Virulence lcsh:Medicine Ileum Biology Toxicology medicine.disease_cause Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Receptor 030304 developmental biology chemotype 0303 health sciences Toll-like receptor Salmonella Typhimurium lipopolysaccharide ∆rfa mutant lcsh:R TLR2 medicine.anatomical_structure chemistry germ-free TLR4 piglet lipids (amino acids peptides and proteins) 030217 neurology & neurosurgery |
| Zdroj: | Toxins, Vol 12, Iss 545, p 545 (2020) Toxins Volume 12 Issue 9 |
| ISSN: | 2072-6651 |
| Popis: | Salmonella Typhimurium is a Gram-negative bacterium that causes enterocolitis in humans and pigs. Lipopolysaccharide (LPS) is a component of the outer leaflet of Gram-negative bacteria that provokes endotoxin shock. LPS can be synthesized completely or incompletely and creates S (smooth) or R (rough) chemotypes. Toll-like receptors (TLR) 2, 4, and 9 initiate an inflammatory reaction to combat bacterial infections. We associated/challenged one-week-old gnotobiotic piglets with wild-type S. Typhimurium with S chemotype or its isogenic ∆rfa mutants with R chemotype LPS. The wild-type S. Typhimurium induced TLR2 and TLR4 mRNA expression but not TLR9 mRNA expression in the ileum and colon of one-week-old gnotobiotic piglets 24 h after challenge. The TLR2 and TLR4 stimulatory effects of the S. Typhimurium ∆rfa mutants were related to the completeness of their LPS chain. The transcription of IL-12/23 p40, IFN-&gamma and IL-6 in the intestine and the intestinal and plasmatic levels of IL-12/23 p40 and IL-6 but not IFN-&gamma were related to the activation of TLR2 and TLR4 signaling pathways. The avirulent S. Typhimurium ∆rfa mutants are potentially useful for modulation of the TLR2 and TLR4 signaling pathways to protect the immunocompromised gnotobiotic piglets against subsequent infection with the virulent S. Typhimurium. |
| Databáze: | OpenAIRE |
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