The effect of telomerase activity on vascular smooth muscle cell proliferation in type 2 diabetes in vivo and in vitro
| Autor: | Zhibin Cao, Ningning Hou, Fang Han, Junling Yi, Xiaodong Sun |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Blood Glucose
Male Cancer Research medicine.medical_specialty Telomerase Vascular smooth muscle medicine.medical_treatment Myocytes Smooth Muscle Type 2 diabetes Biology Biochemistry Muscle Smooth Vascular Diabetes Mellitus Experimental Downregulation and upregulation Internal medicine Diabetes mellitus Genetics medicine Hyperinsulinemia Animals Myocyte Rats Wistar Molecular Biology Triglycerides Cell Proliferation Oligoribonucleotides Insulin Fatty Acids medicine.disease Rats Endocrinology Diabetes Mellitus Type 2 Oncology cardiovascular system Molecular Medicine |
| Zdroj: | Molecular Medicine Reports. 7:1636-1640 |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2013.1350 |
| Popis: | Serious complications as a result of type 2 diabetes mellitus (T2DM) are becoming a major health concern. In the present study, it was hypothesized that telomerase activity is upregulated in vascular smooth muscle cells (VSMCs) during proliferation in T2DM and that the application of telomerase inhibitors impedes the proliferation of VSMCs in vitro. Male Wistar rats were randomly allocated into the normal control (NC) or diabetic (DM) group. Diabetes was induced by high‑fat feeding and a low dose of streptozotocin (STZ; 30 mg/kg). Primary VSMC cultures were exposed to normal (5.5 mM) or high (25 mM) glucose and insulin (100 nM) in the presence and absence of various concentrations of antisense oligoribonucleotides (ASODNs) for varying lengths of time. Telomerase activity and the proliferation of VSMCs were measured. Results showed that there was a significant increase in the levels of fasting glucose, insulin, triglycerides (TG) and free fatty acids (FFAs) in the diabetic group. Telomerase activity and the proliferation of VSMCs were significantly higher in the diabetic group in vivo and in the high glucose and insulin (HGI)-treated group in vitro (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|