The endothelial status reflected by circulating endothelial cells, circulating endothelial progenitor cells and soluble thrombomodulin in patients with mild and resistant hypertension

Autor: Bogna Gryszczyńska, Maria Iskra, Beata Begier-Krasińska, Magdalena Paulina Kasprzak, Magdalena Budzyń, Alicja Bukowska, Mariusz Kaczmarek, Angelika Osińska, Maciej Boruczkowski
Rok vydání: 2018
Předmět:
0301 basic medicine
Adult
medicine.medical_specialty
Physiology
Thrombomodulin
Resistant hypertension
Drug Resistance
Inflammation
Blood Pressure
Enzyme-Linked Immunosorbent Assay
030204 cardiovascular system & hematology
Severity of Illness Index
Flow cytometry
03 medical and health sciences
Young Adult
0302 clinical medicine
Predictive Value of Tests
Internal medicine
medicine
Humans
In patient
Endothelial dysfunction
Progenitor cell
Antihypertensive Agents
Aged
Endothelial Progenitor Cells
Pharmacology
medicine.diagnostic_test
business.industry
Middle Aged
medicine.disease
Flow Cytometry
Soluble thrombomodulin
Lipids
030104 developmental biology
Endocrinology
Phenotype
Case-Control Studies
Hypertension
cardiovascular system
Molecular Medicine
Endothelium
Vascular
medicine.symptom
Inflammation Mediators
business
Biomarkers
Zdroj: Vascular pharmacology. 113
ISSN: 1879-3649
Popis: Background The aim of the present study was to evaluate endothelial status by measuring the concentration of novel markers of endothelial dysfunction (ED): a number of circulating endothelial cells (CECs), circulating endothelial progenitor cells (CEPCs) and their ratio (CEPCs/CECs) as well as a traditional parameter - soluble thrombomodulin (sTM) in patients with resistant (RH) and mild hypertension (MH). Materials and methods Thirty patients with MH and thirty subjects with RH were involved in the study. The control group included thirty-three age and sex-matched normotensive volunteers. We used multicolor flow cytometry for CECs and CEPCs analysis and the commercial human sTM ELISA kit to measure plasma sTM concentration. Results An elevated CECs number and a decreased CEPCs/CECs ratio was found in MH as well as in RH patients in comparison with normotensive volunteers. CECs correlated positively with an increased triglycerides in MH patients and an elevated LDL-cholesterol and hsCRP in RH group. Positive correlation between CEPCs and LDL-cholesterol level was observed in both types of hypertension. Conclusions The results of the present study suggest that an endothelial alteration accompanies hypertension. The number of CECs reflecting the extent of endothelial damage does not appear to be related to the severity of disease. The drastically decreased ratio between CEPCs and CECs observed in both groups of patients suggests an inadequate process of endothelial regeneration. Among analyzed factors inflammation and lipid abnormalities may have significant contribution in endothelial pathology in hypertension.
Databáze: OpenAIRE
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