Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethyl sulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability
| Autor: | Jan Richter, Jakub Šebera, Iva Falková, Shelby E. Follett, K. Wade Elliott, Júlia Mičová, David Řeha, Daniel Šimek, Karel Pomeisl, Martin Golan, Irena Kratochvílová, Martin Falk, Silvia Sedláková, Krisztina Varga |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cryoprotectant Dimethyl sulfoxide General Chemical Engineering Context (language use) General Chemistry Trehalose Article 03 medical and health sciences chemistry.chemical_compound Crystallography 030104 developmental biology Differential scanning calorimetry Cryopreserved Cell chemistry Antifreeze protein Biophysics Viability assay |
| Zdroj: | RSC advances. 7(1) |
| ISSN: | 2046-2069 |
| Popis: | In this work the physico-chemical properties of selected cryoprotectants (antifreeze protein TrxA-AFP752, trehalose and dimethyl sulfoxide) were correlated with their impact on the constitution of ice and influence on frozen/thawed cell viability. The freezing processes and states of investigated materials solutions were described and explained from a fundamental point of view using ab initio modelling (molecular dynamics, DFT), Raman spectroscopy, differential scanning calorimetry and X-ray diffraction. For the first time, in this work we correlated the microscopic view (modelling) with the description of the frozen solution states and put these results in the context of human skin fibroblast viability after freezing and thawing. DMSO and AFP had different impacts on their solution's freezing process but in both cases the ice crystallinity size was considerably reduced. DMSO and AFP treatment in different ways improved the viability of frozen/thawed cells. |
| Databáze: | OpenAIRE |
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