Effects of glutamine, taurine and their association on inflammatory pathway markers in macrophages
| Autor: | Guilherme Galvão dos Santos, Amanda Nogueira-Pedro, Primavera Borelli, Ricardo Ambrósio Fock, Talita Sartori, Marcelo Macedo Rogero, Edson Naoto Makiyama |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Taurine Lipopolysaccharide Cell Survival Glutamine Immunology Biology CITOCINAS 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Immune system Animals Pharmacology (medical) Viability assay Pharmacology chemistry.chemical_classification Dose-Response Relationship Drug Macrophages Drug Synergism Cell cycle Amino acid Cell biology IκBα 030104 developmental biology RAW 264.7 Cells Biochemistry chemistry 030220 oncology & carcinogenesis Inflammation Mediators |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | The immune system is essential for the control and elimination of infections, and macrophages are cells that act as important players in orchestrating the various parts of the inflammatory/immune response. Amino acids play important role in mediating functionality of the inflammatory response, especially mediating macrophages functions and cytokines production. We investigated the influence of glutamine, taurine and their association on the modulation of inflammatory pathway markers in macrophages. The RAW 264.7 macrophage cell line was cultivated in the presence of glutamine and taurine and proliferation rates, cell viability, cell cycle phases, IL-1α, IL-6, IL-10 and TNF-α as well as H2O2 production and the expression of the transcription factor, NFκB, and its inhibitor, IκBα, were evaluated. Our results showed an increase in viable cells and increased proliferation rates of cells treated with glutamine concentrations over 2 mM, as well as cells treated with both glutamine and taurine. The cell cycle showed a higher percentage of cells in the phases S, G2 and M when they were treated with 2 or 10 mM glutamine, or with glutamine and taurine in cells stimulated with lipopolysaccharide. The pNFκB/NFκB showed reduced ratio expression when cells were treated with 10 mM of glutamine or with glutamine in association with taurine. These conditions also resulted in reduced TNF-α, IL-1α and H2O2 production, and higher production of IL-10. These findings demonstrate that glutamine and taurine are able to modulate macrophages inflammatory pathways, and that taurine can potentiate the effects of glutamine, illustrating their immunomodulatory properties. |
| Databáze: | OpenAIRE |
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