AF4-UV-ICP-MS for detection and quantification of silver nanoparticles in seafood after enzymatic hydrolysis
| Autor: | Pilar Bermejo-Barrera, Susana Cuello-Nuñez, Antonio Moreda-Piñeiro, María Vanesa Taboada-López, Heidi Goenaga-Infante, Dorota Bartczak |
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| Rok vydání: | 2021 |
| Předmět: |
Silver
Aqueous solution Chromatography Chemistry Elution Hydrolysis Spectrum Analysis 010401 analytical chemistry Metal Nanoparticles Regenerated cellulose 02 engineering and technology Fractionation 021001 nanoscience & nanotechnology 01 natural sciences Fractionation Field Flow Mass Spectrometry Silver nanoparticle 0104 chemical sciences Analytical Chemistry Seafood Enzymatic hydrolysis Particle Size 0210 nano-technology Inductively coupled plasma mass spectrometry |
| Zdroj: | Talanta. 232:122504 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2021.122504 |
| Popis: | A method based on asymmetric flow field-flow fractionation (AF4) coupled to ultraviolet–visible (UV–vis) spectroscopy and inductively coupled plasma mass spectrometry (ICP-MS) has been developed for silver nanoparticles (Ag NPs) detection and quantification in bivalve molluscs. Samples were pre-treated using a conventional enzymatic (pancreatin and lipase) hydrolysis procedure (37 °C, 12 h). AF4 was performed using a regenerated cellulose (RC) membrane (10 kDa, 350 μm spacer) and aqueous 5 mM Tris-HCl pH = 7.4 as carrier. AF4 separation was achieved with a program that included a focusing step with tip and focus flows of 0.20 and 3.0 mL min−1, respectively, and an injection time of 4.0 min. Elution of different size fractions was performed using a cross flow of 3.0 mL min−1 for 15 min, followed by linear cross flow decrease for 7.5 min, and a washing step for 9.4 min with no cross flow. Several bivalve molluscs (clams, oysters and variegated scallops) were analysed for total Ag content (ICP-MS after microwave assisted acid digestion), and for Ag NPs by the method presented here. Results show that Ag NPs are detected at the same elution time than proteins (UV monitoring at 280 and 405 nm), which suggests a certain interaction occurred between Ag NPs with proteins in the enzymatic extracts. AF4-UV-ICP-MS fractograms also suggest different Ag NPs size distributions for selected samples. Membrane recoveries, determined by peak area comparison of fractograms with and without application of cross flow, were within the 49–121% range. Confirmation of the presence Ag NPs in the investigated enzymatic extracts was demonstrated by SEM after an oxidative pre-treatment based on hydrogen peroxide and microwave irradiation. |
| Databáze: | OpenAIRE |
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