Intraspinal Grafting of Serotonergic Neurons Modifies Expression of Genes Important for Functional Recovery in Paraplegic Rats
| Autor: | Malgorzata Zawadzka, Krzysztof Miazga, Hanna Fabczak, Larry M. Jordan, Anna Bejrowska, Marek Bekisz, Ewa Joachimiak, Łucja Krzemień-Ojak, Urszula Sławińska |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Article Subject Cell Transplantation Gene Expression Hindlimb Biology Serotonergic Thoracic Vertebrae lcsh:RC321-571 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Fetal Tissue Transplantation Internal medicine Gene expression medicine Animals Receptor Serotonin 5-HT2A Gliosis Rats Wistar Muscle Skeletal Receptor lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Spinal Cord Injuries Motor Neurons Paraplegia Recovery of Function Spinal cord Transplantation 030104 developmental biology medicine.anatomical_structure Endocrinology Neurology Receptors Serotonin Female Neurology (clinical) Serotonin Locomotion 030217 neurology & neurosurgery Research Article Serotonergic Neurons |
| Zdroj: | Neural Plasticity Neural Plasticity, Vol 2018 (2018) |
| ISSN: | 1687-5443 2090-5904 |
| DOI: | 10.1155/2018/4232706 |
| Popis: | Serotonin (5-hydroxytryptamine; 5-HT) plays an important role in control of locomotion, partly through direct effects on motoneurons. Spinal cord complete transection (SCI) results in changes in 5-HT receptors on motoneurons that influence functional recovery. Activation of 5-HT2Aand 5-HT7receptors improves locomotor hindlimb movements in paraplegic rats. Here, we analyzed the mRNA of 5-HT2Aand 5-HT7receptors (encoded byHtr2aandHtr7genes, resp.) in motoneurons innervating tibialis anterior (TA) and gastrocnemius lateralis (GM) hindlimb muscles and the tail extensor caudae medialis (ECM) muscle in intact as well as spinal rats. Moreover, the effect of intraspinal grafting of serotonergic neurons onHtr2aandHtr7gene expression was examined to test the possibility that the graft origin 5-HT innervation in the spinal cord of paraplegic rats could reverse changes in gene expression induced by SCI. Our results indicate that SCI at the thoracic level leads to changes inHtr2aandHtr7gene expression, whereas transplantation of embryonic serotonergic neurons modifies these changes in motoneurons innervating hindlimb muscles but not those innervating tail muscles. This suggests that the upregulation of genes critical for locomotor recovery, resulting in limb motoneuron plasticity, might account for the improved locomotion in grafted animals. |
| Databáze: | OpenAIRE |
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