Impact of Zinc- or Copper-Doped Mesoporous Bioactive Glass Nanoparticles on the Osteogenic Differentiation and Matrix Formation of Mesenchymal Stromal Cells

Autor:	Sebastian Wilkesmann, Qaisar Nawaz, Aldo R. Boccaccini, Felix Rehder, Fabian Westhauser, Elke Kunisch, Simon Decker, Arash Moghaddam
Rok vydání:	2021
Předmět:	mesoporous bioactive glass nanoparticles extracellular matrix 0206 medical engineering Nanoparticle chemistry.chemical_element osteogenic differentiation 02 engineering and technology Zinc Matrix (biology) lcsh:Technology Article law.invention Extracellular matrix law medicine General Materials Science Viability assay lcsh:Microscopy lcsh:QC120-168.85 lcsh:QH201-278.5 lcsh:T Chemistry Mesenchymal stem cell zinc 021001 nanoscience & nanotechnology medicine.disease 020601 biomedical engineering lcsh:TA1-2040 Bioactive glass copper Biophysics lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering lcsh:Engineering (General). Civil engineering (General) 0210 nano-technology lcsh:TK1-9971 ddc:666 Calcification
Zdroj:	Materials Volume 14 Issue 8 Materials, Vol 14, Iss 1864, p 1864 (2021)
ISSN:	1996-1944
Popis:	Mesoporous bioactive glass nanoparticles (MBGNs) have gained relevance in bone tissue engineering, especially since they can be used as vectors for therapeutically active ions like zinc (Zn) or copper (Cu). In this study, the osteogenic properties of the ionic dissolution products (IDPs) of undoped MBGNs (composition in mol%: 70 SiO2, 30 CaO) and MBGNs doped with 5 mol% of either Zn (5Zn-MBGNs) or Cu (5Cu-MBGNs compositions in mol%: 70 SiO2, 25 CaO, 5 ZnO/CuO) on human bone marrow-derived mesenchymal stromal cells were evaluated. Extracellular matrix (ECM) formation and calcification were assessed, as well as the IDPs’ influence on viability, cellular osteogenic differentiation and the expression of genes encoding for relevant members of the ECM. The IDPs of undoped MBGNs and 5Zn-MBGNs had a comparable influence on cell viability, while it was enhanced by IDPs of 5Cu-MBGNs compared to the other MBGNs. IDPs of 5Cu-MBGNs had slightly positive effects on ECM formation and calcification. 5Zn-MBGNs provided the most favorable pro-osteogenic properties since they increased not only cellular osteogenic differentiation and ECM-related gene expression but also ECM formation and calcification significantly. Future studies should analyze other relevant properties of MBGNs, such as their impact on angiogenesis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e87f6b26277417affb2970a5c9ef4ab https://pubmed.ncbi.nlm.nih.gov/33918612 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.