A third purine biosynthetic pathway encoded by aminoadenine-based viral DNA genomes
| Autor: | Ahmed Haouz, Simonetta Gribaldo, Pierre Simon Garcia, Najwa Taib, Pascal Röthlisberger, Pierre Alexandre Kaminski, Marion Lagune, Philippe Marliere, Jérôme Loc’h, Dona Sleiman |
|---|---|
| Přispěvatelé: | Biologie des Bactéries pathogènes à Gram-positif - Biology of Gram-Positive Pathogens, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Biologie Evolutive de la Cellule Microbienne - Evolutionary Biology of the Microbial Cell, Adaptation au stress et Métabolisme chez les entérobactéries - Stress adaptation and metabolism in enterobacteria (SAMe), Dynamique structurale des Macromolécules / Structural Dynamics of Macromolecules, Cristallographie (Plateforme) - Crystallography (Platform), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Chimie bioorganique des acides nucléiques - Bioorganic chemistry of nucleic acids, Génomique métabolique (UMR 8030), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), D.S. and P.S.G. were supported by an ERASynBio 'TNA episome' and by an ERACoBioTech 'Iron Plug’n Play' fellowship, respectively. S.G. acknowledges funding from the French National Agency for research grant Archaevol (ANR-16-CE02-0005-01). This study was funded from the French government’s Investissement d’Avenir program, Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases (grant no. ANR-10-LABX-62-IBEID)., ANR-16-CE02-0005,Arch-Evol,Approches phylogenomiques pour étudier l'origine et évolution des Archées(2016), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE) |
| Rok vydání: | 2020 |
| Předmět: |
Purine
DNA polymerase Guanine Genome Viral Viral Nonstructural Proteins Crystallography X-Ray Genome 03 medical and health sciences chemistry.chemical_compound Adenylosuccinate Synthase MESH: 2-Aminopurine A-DNA MESH: Adenylosuccinate Synthase Bacteriophages MESH: Bacteriophages [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] MESH: Phylogeny 2-Aminopurine Phylogeny 030304 developmental biology chemistry.chemical_classification 0303 health sciences Multidisciplinary biology ATP synthase [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] 030302 biochemistry & molecular biology MESH: Crystallography X-Ray MESH: DNA Viral Biosynthetic Pathways Enzyme chemistry Biochemistry DNA Viral biology.protein MESH: Viral Nonstructural Proteins MESH: Biosynthetic Pathways MESH: Genome Viral DNA |
| Zdroj: | Science Science, 2021, 372 (6541), pp.516-520. ⟨10.1126/science.abe6494⟩ Science, American Association for the Advancement of Science, 2021, 372 (6541), pp.516-520. ⟨10.1126/science.abe6494⟩ |
| ISSN: | 1095-9203 0036-8075 |
| Popis: | Biosynthesis and replication, from A to Z Four nucleobases. adenine (A), cytosine (C), guanine (G), and thymine (T), are usually thought to be invariable in DNA. In bacterial viruses, however, each of the DNA bases have variations that help them to escape degradation by bacterial restriction enzymes. In the genome of cyanophage S-2L, A is completely replaced by diaminopurine (Z), which forms three hydrogen bonds with T and thus creates non–Watson-Crick base pairing in the DNA of this virus (see the Perspective by Grome and Isaacs). Zhou et al. and Sleiman et al. determined the biochemical pathway that produces Z, which revealed more Z genomes in viruses hosted in bacteria distributed widely in the environment and phylogeny. Pezo et al. identified a DNA polymerase that incorporates Z into DNA while rejecting A. These findings enrich our understanding of biodiversity and expand the genetic palette for synthetic biology. Science , this issue p. 512 , 516 , 520 ; see also p. 460 |
| Databáze: | OpenAIRE |
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