Antiproliferative Activity of Neem Leaf Extracts Obtained by a Sequential Pressurized Liquid Extraction
| Autor: | Cláudio Dariva, Andriele Mendonça Barbosa, Klebson Silva Santos, Isabel C.F.R. Ferreira, Elton Franceschi, Ricardo C. Calhelha, Marcelo da Costa Mendonça, Francine Ferreira Padilha, Victor de Freitas, Maria Beatriz P.P. Oliveira, Ana Veruska Cruz da Silva Muniz |
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| Přispěvatelé: | ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC. |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
antiproliferative activity
sequential pressurized liquid extraction Nim indiano Ethyl acetate lcsh:Medicine lcsh:RS1-441 Pharmaceutical Science Antiproliferative activity 01 natural sciences Article Extractor lcsh:Pharmacy and materia medica Terpene HeLa chemistry.chemical_compound Drug Discovery parasitic diseases Sequential pressurized liquid extraction Ethanol Chromatography biology 010405 organic chemistry Chemistry lcsh:R 010401 analytical chemistry Extraction (chemistry) Azadirachta biology.organism_classification 0104 chemical sciences Hexane neem leaves Molecular Medicine Neem leaves Extrato |
| Zdroj: | Pharmaceuticals Volume 11 Issue 3 Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Pharmaceuticals, Vol 11, Iss 3, p 76 (2018) |
| ISSN: | 1424-8247 |
| DOI: | 10.3390/ph11030076 |
| Popis: | Azadirachta indica A. Juss (neem) extracts have been used in pharmaceutical applications as antitumor agents, due to their terpenes and phenolic compounds. To obtain extracts from neem leaves with potential antiproliferative effect, a sequential process of pressurized liquid extraction was carried out in a fixed bed extractor at 25 ° C and 100 bar, using hexane (SH), ethyl acetate (SEA), and ethanol (SE) as solvents. Extractions using only ethanol (EE) was also conducted to compare the characteristics of the fractionated extracts. The results obtained by liquid chromatography-electrospray ionization mass spectrometry suggested a higher concentration of terpenes in the SEA extract in comparison to SH, SE, and EE extracts. Therefore, antiproliferative activity showed that SEA extracts were the most efficient inhibitor to human tumor cells MCF-7, NCI-H460, HeLa, and HepG2. Hepatocellular cells were more resistant to SH, SEA, SE, and EE compared to breast, lung, hepatocellular, and cervical malignant cells. Neem fractioned extracts obtained in the present study seem to be more selective for malignant cells compared to the non-tumor cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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