Isolation and Characterization of High Affinity Aptamers Against DNA Polymerase Iota
| Autor: | A. S. Efremova, L. V. Gening, Viacheslav Z. Tarantul, Alena V. Makarova, A. V. Lakhin, Yuri I. Pavlov, S. I. Shram, Andrei A. Kazakov |
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| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
Base Sequence biology Aptamer SELEX Aptamer Technique Context (language use) DNA-Directed DNA Polymerase Processivity Aptamers Nucleotide Biochemistry Molecular biology Enzyme chemistry DNA Polymerase iota Drug Discovery Genetics biology.protein Humans Molecular Medicine DNA Polymerase Iota Molecular Biology Polymerase Systematic evolution of ligands by exponential enrichment DNA Primers |
| Zdroj: | Nucleic Acid Therapeutics. 22:49-57 |
| ISSN: | 2159-3345 2159-3337 |
| DOI: | 10.1089/nat.2011.0324 |
| Popis: | Human DNA-polymerase iota (Pol ι) is an extremely error-prone enzyme and the fidelity depends on the sequence context of the template. Using the in vitro systematic evolution of ligands by exponential enrichment (SELEX) procedure, we obtained an oligoribonucleotide with a high affinity to human Pol ι, named aptamer IKL5. We determined its dissociation constant with homogenous preparation of Pol ι and predicted its putative secondary structure. The aptamer IKL5 specifically inhibits DNA-polymerase activity of the purified enzyme Pol ι, but did not inhibit the DNA-polymerase activities of human DNA polymerases beta and kappa. IKL5 suppressed the error-prone DNA-polymerase activity of Pol ι also in cellular extracts of the tumor cell line SKOV-3. The aptamer IKL5 is useful for studies of the biological role of Pol ι and as a potential drug to suppress the increase of the activity of this enzyme in malignant cells. |
| Databáze: | OpenAIRE |
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