Adhesion of synchronized human hematopoietic progenitor cells to fibronectin and vascular cell adhesion molecule-1 fluctuates reversibly during cell cycle transit in ex vivo culture
| Autor: | Vincent Artisien, Olivier Giet, Yves Beguin, Sandra Huygen, Ivano Di Stefano, André Gothot |
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| Rok vydání: | 2002 |
| Předmět: |
Time Factors
Immunology Integrin Cell Culture Techniques Vascular Cell Adhesion Molecule-1 Antigens CD34 Biology Biochemistry Antigens CD Cell Adhesion Humans Progenitor cell Cell adhesion Cells Cultured Cell adhesion molecule Cell Cycle Infant Newborn Cell Biology Hematology Delivery Obstetric Fetal Blood Hematopoietic Stem Cells Cell biology Fibronectins Fibronectin biology.protein Neural cell adhesion molecule Stem cell Ex vivo |
| Zdroj: | Blood. 100(8) |
| ISSN: | 0006-4971 |
| Popis: | Ex vivo expansion of hematopoietic stem/progenitor cells may result in defective engraftment. Human cord blood CD34(+) progenitor cells were synchronized and assayed for adhesion and migration onto fibronectin (Fn) and vascular cell adhesion molecule-1 (VCAM-1) at different stages of a first cell cycle executed ex vivo. During S phase transit, adhesion to Fn was transiently increased while binding to VCAM-1 was reversibly decreased, after which adhesion to both ligands returned to baseline levels with cell cycle completion. Transmigration across Fn and VCAM-1 decreased irreversibly during S phase progression. The function of alpha4 and alpha5 integrins was assessed with specific neutralizing antibodies. In uncultured CD34(+) cells and long-term culture-initiating cells (LTC-ICs), both adhesion and migration on Fn were inhibited by anti-alpha4 but not by anti-alpha5 antibodies. In mitotically activated CD34(+) cells and LTC-ICs, adhesion and migration on Fn were mainly dependent on alpha5 integrin and to a lesser extent on alpha4 integrin. Changes in integrin function were not dependent on parallel modulation of integrin expression. In conclusion, Fn and VCAM-1 binding of progenitor cells fluctuates reversibly during cell cycle transit ex vivo. In addition, our data show that mitogenic activation induces a shift from a dominant alpha4 to a preferential alpha5 integrin-dependent interaction with Fn. |
| Databáze: | OpenAIRE |
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