Femtosecond laser nano/micro patterning of titanium influences mesenchymal stem cell adhesion and commitment
| Autor: | Jean-Claude Dumas, Julien Granier, Aline Rattner, Ximena Zapata, Laurence Vico, Hassan Zahouani, Wafa Bouleftour, Virginie Dumas, Cyril Mauclair, Alain Guignandon, Marie Thérèse Linossier, Sylvie Peyroche |
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| Přispěvatelé: | Laboratoire de Tribologie et Dynamique des Systèmes (LTDS), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Ecole Nationale d'Ingénieurs de Saint Etienne-Centre National de la Recherche Scientifique (CNRS), Contraintes mécaniques et tissu osseux, Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biologie intégrative du tissu osseux, Laboratoire Hubert Curien [Saint Etienne] (LHC), Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), Grupo de Immunovirologia, Corporacion Biogenesis, Universidad de Antoquia, GIE Manutech-USD |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Materials science
Surface Properties Biomedical Engineering chemistry.chemical_element Biocompatible Materials Bioengineering Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Cell Line Biomaterials Focal adhesion Mice Cell Movement Osteogenesis Materials Testing Cell Adhesion Animals Texture (crystalline) Cell adhesion ComputingMilieux_MISCELLANEOUS Titanium Adipogenesis Lasers Mesenchymal stem cell technology industry and agriculture Mesenchymal Stem Cells Adhesion 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Femtosecond [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic Surface modification 0210 nano-technology Biomedical engineering |
| Zdroj: | Biomedical Materials Biomedical Materials, IOP Publishing, 2015, 10 (5), pp.055002. ⟨10.1088/1748-6041/10/5/055002⟩ IOP Publishing |
| ISSN: | 1748-6041 1748-605X |
| Popis: | Surface improvement of implants is essential for achieving a fast osseo-integration. Technically, the creation of a precise pattern on a titanium alloy surface is challenging. Here, the femtosecond laser was chosen as an innovative technology for texturing with accuracy a nano-micro topography. By adjusting the laser parameters, three biomimetic textures were fabricated on the titanium surface: micropits with nano-ripples in the pits, micropits with nano-ripples around the pits, and a texture with only nano-ripples. Mesenchymal stem cells (MSCs, C3H10T1/2) grown on these surfaces displayed altered morphometric parameters, and modified their focal adhesions in term of number, size, and distribution depending on surface type. These results indicate that the MSCs perceived subtle differences in topography. Dynamic analyses of early cellular events showed a higher speed of spreading on all the textured surfaces as opposed to the polished titanium. Concerning commitment, all the laser-treated surfaces strongly inhibited the expression of adipogenic-related genes (PPARϒ2, C/EBPα) and up-regulated the expression of osteoblastic-related genes (RUNX2, osteocalcin). Interestingly, the combination of micropits to nano-ripples enhanced their osteogenic potential as seen by a twofold increase in osteocalcin mRNA. Alkaline phosphatase activity was increased on all the textured surfaces, and lipid production was down-regulated. The functionalization of metallic surfaces by this high-resolution process will help us understand the MSCs' interactions with substrates for the development of textured implants with predictable tissue integrative properties. |
| Databáze: | OpenAIRE |
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