Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: finding new anti-inflammatory immunobiotics

Autor: Julio Villena, Masanori Tohno, Jin-zhong Xiao, Haruki Kitazawa, Takeshi Shimosato, Tomoyuki Shimazu, Noriyuki Iwabuchi, Kyoko Morie, Muneo Numasaki, Tadao Saito, Yoshihito Suda, Keiji Iwatsuki, Hitomi Fujie, Tomoko Yaeshima, Hisashi Aso
Rok vydání: 2011
Předmět:
Microbiology (medical)
INTESTINAL INFLAMMATION
Otras Biotecnología Agropecuaria
Swine
Biotecnología Agropecuaria
Immunology
Cell
ved/biology.organism_classification_rank.species
Biology
Real-Time Polymerase Chain Reaction
medicine.disease_cause
Microbiology
Proinflammatory cytokine
Immune system
Genes
Reporter
Enterotoxigenic Escherichia coli
Escherichia coli
medicine
Animals
Immunology and Allergy
Intestinal Mucosa
Cells
Cultured
Inflammation
Toll-like receptor
Bifidobacterium breve
PORCINE INTESTINAL EPITHELILA CELLS
ved/biology
Gene Expression Profiling
TLR-2 ACTIVATION
HEK 293 cells
Epithelial Cells
General Medicine
Flow Cytometry
Coculture Techniques
Toll-Like Receptor 2
Infectious Diseases
medicine.anatomical_structure
ANTI-INFLAMMATORY BIFIDOBACTERIA
CIENCIAS AGRÍCOLAS
Cell culture
Cytokines
Bifidobacterium
Zdroj: FEMS Immunology & Medical Microbiology. 63:129-139
ISSN: 1574-695X
0928-8244
DOI: 10.1111/j.1574-695x.2011.00837.x
Popis: A total of 23 strains of bifidobacteria taxonomically belonging to five species were tested for their potent immunomodulatory effect using a combination of two methods: the NF-κB-reporter assay using a toll-like receptor 2-expressing transfectant (HEK pTLR2 system) and the mitogenic assay using porcine Peyer's patches immunocompetent cells. Among the four preselected strains from different immunomodulatory groups, Bifidobacterium breve MCC-117 was able to efficiently modulate the inflammatory response triggered by enterotoxigenic Escherichia coli (ETEC) in a porcine intestinal epithelial (PIE) cell line. Moreover, using PIE cells and swine Peyer's patches immunocompetent cell co-culture system, we demonstrated that the immunoregulatory effect of B. breve MCC-117 was related to the capacity of the strain to influence PIE and immune cell interactions, leading to the stimulation of regulatory T cells. The results suggested that bifidobacteria that express high activity in both the HEK pTLR2 and the mitogenic assays may behave like potential anti-inflammatory strains. The combination of the HEK pTLR2 system, the evaluation of mitogenic activity and PIE cells will be of value for the development of new immunologically functional foods and feeds that could prevent inflammatory intestinal disorders. Although our findings should be proven in appropriate experiments in vivo, the results of the present work provide a scientific rationale for the use of B. breve MCC-117 to prevent ETEC-induced intestinal inflammation. Fil: Fujie, Hitomi. Tohoku University; Japón Fil: Villena, Julio Cesar. Tohoku University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Tohno, Masanori. National Agricultural Research Organization. National Institute of Livestock and Grassland Science. Functional Feed Research Team; Japón Fil: Morie, Kyoko. Tohoku University; Japón Fil: Shimazu, Tomoyuki. Tohoku University; Japón Fil: Aso, Hisashi. Tohoku University; Japón Fil: Suda, Yoshihito. Miyagi University; Japón Fil: Shimosato, Takeshi. Shinshu University; Japón Fil: Iwabuchi, Noriyuki. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón Fil: Xiao, Jin-Zhong. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón Fil: Yaeshima, Tomoko. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón Fil: Iwatsuki, Keiji. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón Fil: Saito, Tadao. Tohoku University; Japón Fil: Numasaki, Muneo. Josai University; Japón Fil: Kitazawa, Haruki. Tohoku University; Japón
Databáze: OpenAIRE
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