Ionic calcium content of light dense human red cells separated by percoll density gradients
| Autor: | Eneida A. Romero, Moraima D Winkler B, Pedro J. Romero |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Erythrocytes
Potassium Biophysics chemistry.chemical_element Cell Count Light cell Cell Separation Glucosephosphate Dehydrogenase Biochemistry Hemoglobins Viscosity Adenosine Triphosphate Red cell sub-population Centrifugation Density Gradient Humans Free Ca2+ content Light Cell Dense cell Colloids Percoll gradient Chromatography Red Cell Chemistry Mean value Povidone Erythrocyte Aging Cell Biology Creatine Silicon Dioxide Erythrocyte (Human) Evaluation Studies as Topic Content (measure theory) Acetylcholinesterase Ionic calcium Calcium Percoll Biomarkers |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 1323(1):23-28 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/s0005-2736(96)00141-1 |
| Popis: | In this paper we have compared the adequacy of two methods using Percoll density gradients to separate light and dense erythrocytes from fresh human blood. After measuring the distribution of some classical age-markers such as haemoglobin, potassium and creatine contents, it was found that preformed gradients generated more stringent conditions for age-related density separations. Employing such gradients, the free Ca 2q content of above sub-populations was assessed with Fura-2, 2q . under conditions where the viscosity effect was abolished. In five experiments, the free Ca content mean value " 1 S.D. was 8.4 " 2.82 nM and 31.2" 13.0 nM for the 7-10% lightest and densest cells, respectively. These results are discussed in connection to red cell senescence. 2q . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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