Antifungal activity of stilbenes in in vitro bioassays and in transgenic Populus expressing a gene encoding pinosylvin synthase
| Autor: | Sanna-Kaisa Seppänen, Ari Pappinen, K. von Weissenberg, L. Syrjälä, L Paajanen, Teemu H. Teeri |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Phellinus tremulae
Antifungal Agents Agrobacterium pinosylvin Pinosylvin Gene Expression Pinosylvin synthase Plant Science Genetically modified crops stilbenes resveratrol Resveratrol chemistry.chemical_compound Transformation Genetic Botany Stilbenes decay fungi biology transformation fungi Fungi General Medicine biology.organism_classification Plants Genetically Modified Enzyme assay Transformation (genetics) Populus chemistry Agrobacterium tumefaciens biology.protein Agronomy and Crop Science Acyltransferases |
| Zdroj: | Seppänen, S K, Syrjälä, L, von Weissenberg, K, Teeri, T H, Paajanen, L & Pappinen, A 2004, ' Antifungal activity of stilbenes in in vitro bioassays and in transgenic Populus expressing a gene encoding pinosylvin synthase ', Plant Cell Reports, vol. 22, no. 8, pp. 584-593 . https://doi.org/10.1007/s00299-003-0728-0 |
| ISSN: | 0721-7714 |
| DOI: | 10.1007/s00299-003-0728-0 |
| Popis: | The effect of two stilbene compounds, pinosylvin and resveratrol, on the growth of several fungi was evaluated in plate tests. Wood decay tests were carried out with birch and aspen samples impregnated with the two stilbenes. In plate experiments, resveratrol had an enhancing effect on growth at concentrations where pinosylvin was already enough to prevent the growth of most fungi studied. Pinosylvin impregnated at 0.2% (w/w) concentration significantly reduced the decay caused by all fungi except Phellinus tremulae. In contrast, a resveratrol content of 0.8%, did not protect the wood from decay. A pinosylvin-synthase-encoding gene from Pinus sylvestris was transferred into aspen (Populus tremula) and two hybrid aspen clones (Populus tremula×tremuloides) by Agrobacterium tumefaciens-mediated transformation. Transgenic plants accumulated pinosylvin synthase-specific mRNA and showed stilbene synthase enzyme activity in vitro. Transgenic aspen line H4 showed increased resistance to Phellinus tremulae, while two hybrid aspen transformants decayed faster than the control trees. However, we were unable to detect the accumulation of stilbenes in the transgenic plantlets. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink