In planta transcriptome analysis reveals tissue-specific expression of pathogenicity genes and microRNAs during rice-Magnaporthe interactions
| Autor: | Guo-Liang Wang, Meghana Deepak Shirke, H. B. Mahesh, Malali Gowda |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Magnaporthe Genes Fungal Genes Plant 01 natural sciences Transcriptome 03 medical and health sciences Ascomycota microRNA Genetics Pathogen Gene Disease Resistance 030304 developmental biology 0303 health sciences biology MicroRNA sequencing food and beverages Cytochrome P450 Oryza Isocitrate lyase biology.organism_classification Plant Leaves MicroRNAs biology.protein 010606 plant biology & botany |
| Zdroj: | Genomics. 113:265-275 |
| ISSN: | 0888-7543 |
| Popis: | Transcriptional re-programming in host and pathogen upon leaf and neck infection is an evolving area of research for the rice blast community. Analysis of in planta rice transcriptome in leaf and neck tissues revealed tissue-specific and infection-specific expression of rice and Magnaporthe oryzae genes in host and pathogen. The glycosyl hydrolase, isocitrate lyase, cupin domain containing protein, TF2, CMPG1, CHIT17 and OsCML14 genes were uniquely expressed in leaf infection. Genes like cytochrome P450, inhibitor I family protein, GSTU6, abscisic stress ripening, and cupin domain containing protein were up-regulated during neck infection. In our microRNA sequencing study, Osa-miR166n-3p was highly expressed in upon Magnaporthe leaf infection, whereas osa-miR1661-3p, osa-miR166n-3p and osa-miR159b were overexpressed in neck infection. Here we report several transcripts being targeted by up and down regulated microRNAs during infection. The putative genes expressed upon infection in leaf and neck could be used in understanding the dual-epidemics of blast disease. |
| Databáze: | OpenAIRE |
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