The Lr34 adult plant rust resistance gene provides seedling resistance in durum wheat without senescence
| Autor: | Davinder Singh, Rainer Boni, Simon G. Krattinger, Robert F. Park, Michael Ayliffe, Evans Lagudah, Brian Gilbert, Amy Rinaldo |
|---|---|
| Přispěvatelé: | University of Zurich, Ayliffe, Michael |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant Science Genetically modified crops 580 Plants (Botany) Stem rust 01 natural sciences Rust 03 medical and health sciences Wheat leaf rust 10126 Department of Plant and Microbial Biology Botany 1110 Plant Science Puccinia Cultivar 1102 Agronomy and Crop Science Research Articles Triticum Disease Resistance Plant Diseases Plant Proteins biology Basidiomycota Blumeria fungi food and beverages rust Plants Genetically Modified biology.organism_classification Plant Leaves 030104 developmental biology Seedlings Seedling 1305 Biotechnology ABC transporter Agronomy and Crop Science Powdery mildew Research Article 010606 plant biology & botany Biotechnology |
| Zdroj: | Plant Biotechnology Journal |
| Popis: | Summary The hexaploid wheat (Triticum aestivum) adult plant resistance gene, Lr34/Yr18/Sr57/Pm38/Ltn1, provides broad-spectrum resistance to wheat leaf rust (Lr34), stripe rust (Yr18), stem rust (Sr57) and powdery mildew (Pm38) pathogens, and has remained effective in wheat crops for many decades. The partial resistance provided by this gene is only apparent in adult plants and not effective in field-grown seedlings. Lr34 also causes leaf tip necrosis (Ltn1) in mature adult plant leaves when grown under field conditions. This D genome-encoded bread wheat gene was transferred to tetraploid durum wheat (T. turgidum) cultivar Stewart by transformation. Transgenic durum lines were produced with elevated gene expression levels when compared with the endogenous hexaploid gene. Unlike nontransgenic hexaploid and durum control lines, these transgenic plants showed robust seedling resistance to pathogens causing wheat leaf rust, stripe rust and powdery mildew disease. The effectiveness of seedling resistance against each pathogen correlated with the level of transgene expression. No evidence of accelerated leaf necrosis or up-regulation of senescence gene markers was apparent in these seedlings, suggesting senescence is not required for Lr34 resistance, although leaf tip necrosis occurred in mature plant flag leaves. Several abiotic stress-response genes were up-regulated in these seedlings in the absence of rust infection as previously observed in adult plant flag leaves of hexaploid wheat. Increasing day length significantly increased Lr34 seedling resistance. These data demonstrate that expression of a highly durable, broad-spectrum adult plant resistance gene can be modified to provide seedling resistance in durum wheat. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|