Delayed-rectifying potassium channels in mouse peritoneal macrophages: Pharmacological analysis

Autor:	N. G. Roshchina, O. E. Lebedev, Z. I. Krutetskaya
Rok vydání:	1995
Předmět:	medicine.medical_specialty Physiology General Neuroscience Potassium Inactivation kinetics Kinetics chemistry.chemical_element Potassium channel chemistry.chemical_compound Endocrinology chemistry Internal medicine medicine Biophysics Verapamil Arachidonic acid medicine.drug
Zdroj:	ResearcherID
ISSN:	1573-9007 0090-2977
DOI:	10.1007/bf01059992
Popis:	The effects of 4-aminopyridine, verapamil, and 4-bromophenacylbromide (4-BPB) on the kinetics of delayed outward-rectifying potassium currents (I K) were investigated in cultured mouse peritoneal macrophages using a classical whole-cell patch-clamp technique. The outwardI K was completely blocked by 4-aminopyridine at 1.0 mM concentration. Verapamil at the same concentration also blockedI K completely. Lower concentration (50 µM) of verapamil demonstrated only partial blocking action, which was almost fully reversible, and markedly increased the rate ofI K inactivation. The main effect of 4-BPB on the outwardI K was a significant acceleration ofI K activation and inactivation kinetics. It is suggested that this modulation results from a direct effect of 4-BPB on potassium channels or relates to the arachidonic acid cascade.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e7351c63317e10b7faba16fe4614262 https://doi.org/10.1007/bf01059992 Zobrazit plný text záznamu Full text from SpringerLink