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Supplemental Figure 1. Heatmap for top 100 differentially expressed genes with AGL depletion in bladder cancer cell line UMUC3. Supplemental Figure 2. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) for HAS2, RRAGD, VCAN, EREG and TULP3 in T24T cells with or without AGL. Supplemental Figure 3. A,B,C, Monolayer growth of UMUC3 cells with and without AGL knockdown followed by knockdown of HAS2, RRAGD and TULP3. Supplemental Figure 4. A, qRT-PCR for HAS2 in UMUC3 control and AGL knockdown cells using a second siRNA against HAS2 followed by monolayer growth assay. B, qRT-PCR for HAS2 in MGHU4 cells with AGL knockdown. C, qRT-PCR to validate knockdown of HAS2 in MGHU4 control and AGL knockdown cells followed by monolayer growth assay. Supplemental Figure 5. A, HA ELISA for UMUC3 AGL knockdown cells after knockdown of HAS2 using a new siRNA. B, HA ELISA of MGHU4 cells with or without AGL expression after HAS2 knockdown. C, Monolayer growth of T24T cells with and without AGL on treatment with 4MU. D, Monolayer growth of T24T cells with and without AGL on treatment with 4MU and HA. |
