Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous
| Autor: | Javier Raso, Lourdes Morales-Oyervides, Julio C. Montañez, Diederich Aguilar-Machado, Carlota Delso, Juan Manuel Martínez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Histology esterase lcsh:Biotechnology enzymatic activity Biomedical Engineering Bioengineering 02 engineering and technology Esterase 03 medical and health sciences chemistry.chemical_compound Astaxanthin lcsh:TP248.13-248.65 pulsed electric field Food science Incubation Carotenoid Original Research chemistry.chemical_classification Ethanol Extraction (chemistry) Bioengineering and Biotechnology 021001 nanoscience & nanotechnology Yeast astaxanthin Xanthophyllomyces dendrorhous 030104 developmental biology Enzyme chemistry extraction 0210 nano-technology Biotechnology |
| Zdroj: | Zaguán: Repositorio Digital de la Universidad de Zaragoza Universidad de Zaragoza Frontiers in Bioengineering and Biotechnology Zaguán. Repositorio Digital de la Universidad de Zaragoza instname Frontiers in Bioengineering and Biotechnology, Vol 8 (2020) |
| Popis: | The aim of this study was to evaluate the potential of pulsed electric fields (PEF) to improve the extraction of the lipid-soluble astaxanthin from fresh biomass of a wild-type (CECT 11028) and mutant (ATCC 74219) Xanthophyllomyces dendrorhous strain using ethanol as solvent. Inactivation and propidium uptake studies revealed that inactivation is a good index for estimated the proportion of irreversible permeabilized cells when inactivation is higher than 70% in the two strains. Ethanol was ineffective for extracting carotenoids from the PEF-treated cells (20 kV/cm, 135 μs) of the two strains. However, after aqueous incubation of PEF-treated X. dendrorhous ATCC 74219 cells for 12 h, up to 2.4 ± 0.05 mg/g dried weight (d.w.) of carotenoids were extracted in ethanol. From total carotenoid extracted, around 84% corresponded to all-trans astaxanthin. The detection and quantification of esterase activity in the supernatant and the relationship between the percentage of esterase activity quantified and the amount of carotenoids extracted indicate that the extraction of astaxanthin was mediated by enzymatic esterase activity triggered by PEF during incubation. On the other hand, the formation of a large lipid globule into the cytoplasm of PEF-treated X. dendrorhous CECT 11028 cells during aqueous incubation prevented carotenoid extraction. The process developed in this investigation represents a more sustainable and greener method that those previously used for extracting astaxanthin from yeast. |
| Databáze: | OpenAIRE |
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