Claudin-1 as a Biomarker of Cervical Cytology and Histology

Autor: Gábor Sobel, Tímea Szekerczés, Attila Kovács, Balázs Járay, Ádám Galamb, Zsuzsa Schaff, Róbert Koiss, Csaba Jeney, Tamás Székely, Márta Benczik
Rok vydání: 2015
Předmět:
0301 basic medicine
Cancer Research
medicine.medical_specialty
Cytodiagnosis
Concordance
Population
Uterine Cervical Neoplasms
Cervical intraepithelial neoplasia
Pathology and Forensic Medicine
Immunoenzyme Techniques
03 medical and health sciences
0302 clinical medicine
Cytology
Claudin-1
Biomarkers
Tumor
medicine
Humans
Neoplasms
Squamous Cell
education
Papillomaviridae
Early Detection of Cancer
Vaginal Smears
Gynecology
Colposcopy
Cervical cancer
Hungary
education.field_of_study
medicine.diagnostic_test
business.industry
Papillomavirus Infections
Case-control study
General Medicine
Prognosis
Uterine Cervical Dysplasia
medicine.disease
030104 developmental biology
Oncology
Case-Control Studies
030220 oncology & carcinogenesis
Biomarker (medicine)
Female
Neoplasm Grading
business
Follow-Up Studies
Zdroj: Pathology & Oncology Research. 22:179-188
ISSN: 1532-2807
1219-4956
Popis: Several immunochemistry tests are used for triaging human papilloma virus (HPV) and cytology positive cases in cervical cancer screening and as an adjunct test to diagnose cervical cancer. Claudin-1 (CLDN1) protein is a major component of the tight junction, shown to have altered expression in cervical cancer. In this study, value of CLDN1 was analysed as a screening and triage immunochemistry test compared to cytology and HPV testing. A population of 352 women attending colposcopic referral visits resulting in cervical conisation and a second population of 150 women attending routine gynaecological visits with negative cervical cytology were enrolled in a multi-centre clinical study in Hungary. Cytology and HPV (Genoid Full Spectrum HPVAmplification and Detection System) testing were carried out along with immunocytochemistry for CLDN1, and as a reference, using CINtec p16 Cytology Kit. Three different evaluation protocols were used which assessed immunostaining characteristics with or without cytological readings. High correlation observable between p16INK4a and CLDN1 established CLDN1 as a competing marker in cervical cancer. Concordance of CLDN1 immunostaining of cervical intraepithelial neoplasia 2 and above (CIN2+) positives was 84.0 % (73.8–89.3); concordance of CIN2+ negatives was 69.0 % (59.6–75.8). In conclusion, CLDN1 has similar diagnostic potential as p16INK4a, our results established it as a histological and cytological biomarker with the potential to improve the clinical performance of cervical cytology and histology.
Databáze: OpenAIRE
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