DHA supplementation during prenatal ethanol exposure alters the expression of fetal rat liver genes involved in oxidative stress regulation

Autor: Fatemeh Ramezani Kapourchali, Michael N. A. Eskin, Bradley A. Feltham, Xavier Lieben Louis, Miyoung Suh
Rok vydání: 2019
Předmět:
Male
medicine.medical_specialty
Antioxidant
Docosahexaenoic Acids
Physiology
Endocrinology
Diabetes and Metabolism
medicine.medical_treatment
Fatty Acids
Nonesterified
medicine.disease_cause
Antioxidants
Rats
Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
Glutathione Peroxidase GPX1
0302 clinical medicine
Pregnancy
Physiology (medical)
Internal medicine
medicine
Animals
Adverse effect
Gene
030304 developmental biology
Glutathione Peroxidase
0303 health sciences
Fetus
Nutrition and Dietetics
Ethanol
business.industry
Central Nervous System Depressants
Gene Expression Regulation
Developmental
Organ Size
General Medicine
Glutathione
Diet
Rats
Endocrinology
Liver
chemistry
Fetal Alcohol Spectrum Disorders
Docosahexaenoic acid
Prenatal Exposure Delayed Effects
Dietary Supplements
Female
lipids (amino acids
peptides
and proteins)
business
030217 neurology & neurosurgery
Oxidative stress
Zdroj: Applied Physiology, Nutrition, and Metabolism. 44:744-750
ISSN: 1715-5320
1715-5312
DOI: 10.1139/apnm-2018-0580
Popis: Prenatal ethanol (EtOH) exposure is known to induce adverse effects on fetal brain development. Docosahexaenoic acid (DHA) has been shown to alleviate these effects by up-regulating antioxidant mechanisms in the brain. The liver is the first organ to receive enriched blood after placental transport. Therefore, it could be negatively affected by EtOH, but no studies have assessed the effects of DHA on fetal liver. This study examined the effects of maternal DHA intake on DHA status and gene expression of key enzymes of the glutathione antioxidant system in the fetal liver after prenatal EtOH exposure. Pregnant Sprague–Dawley dams were intubated with EtOH for the first 10 days of pregnancy, while being fed a control or DHA-supplemented diet. Fetal livers were collected at gestational day 20, and free fatty acids and phospholipid profile, as well as glutathione reductase (GR) and glutathione peroxidase-1 (GPx1) gene expressions, were assessed. Prenatal EtOH exposure increased fetal liver weight, whereas maternal DHA supplementation decreased fetal liver weight. DHA supplementation increased fetal liver free fatty acid and phospholipid DHA independently of EtOH. GR and GPx1 messenger RNA (mRNA) expressions were significantly increased and decreased, respectively, in the EtOH-exposed group compared with all other groups. Providing DHA normalized GR and GPx1 mRNA expression to control levels. This study shows that maternal DHA supplementation alters the expression of fetal liver genes involved in the glutathione antioxidative system during prenatal EtOH exposure. The fetal liver may play an important role in mitigating the signs and symptoms of fetal alcohol spectrum disorders in affected offspring.
Databáze: OpenAIRE
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