An Evaluation of Four Different Luminescence Immunoassay Systems: CELIA (chemiluminescent immunoassay), SPALT (solid-phase antigen luminescence technique), ILMA (immunoluminometric assay) and ILSA (immunoluminometric labelled second antibody). A critical study of macro solid phases for use in immunoassay Systems, Part III
| Autor: | W. G. Wood, A. Gadow, C. J. Strasburger, J. Haritz, H. Fricke, Peter Christian Scriba, Bettina Tode, Heidi-Susanne Krausz |
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| Rok vydání: | 1984 |
| Předmět: |
Quality Control
Hydrocortisone Macromolecular Substances Pyruvate Kinase education Clinical Biochemistry Radioimmunoassay law.invention Part iii Thyroxine-Binding Proteins Antigen law medicine Animals Humans Sodium Hydroxide Chemiluminescence Immunoassay Immunoradiometric assay Chromatography medicine.diagnostic_test biology Chemistry Biochemistry (medical) Ceruloplasmin General Medicine C-Reactive Protein Liver Ferritins Luminescent Measurements biology.protein Antibody Luminescence Spleen |
| Zdroj: | Clinical Chemistry and Laboratory Medicine. 22 |
| ISSN: | 1437-4331 1434-6621 |
| DOI: | 10.1515/cclm.1984.22.5.349 |
| Popis: | The performance of different solid-phase luminescence immunoassays has been documented using four different assay concepts. These are CELIA (chemiluminescence immunoassay), SPALT (solid-phase antigen luminescence technique), ILMA (immunoluminometric assay) and ILSA (immunoluminometric labelled second-antibody assay). CELIA is analogous to a solid-phase radioimmunoassay and uses a labelled antigen, SPALT and ILSA use a labelled second (species-specific) antibody and ILMA a labelled substance-specific antibody, i.e. analogous to the immunoradiometric assay. Both bioluminescent and chemiluminescent labels have been used. Pyruvate kinase was used for bioluminescence and diazoluminol and N-(4-amino-butyl)-N-ethyl isoluminol hemisuccinamide for chemiluminescence. Relevant quality-control parameters and reference ranges have been given for the optimised assays. Assays described are: thyroxine, thyroxine binding globulin, cortisol, caeruloplasmin, ferritin and C-reactive protein. Luminescence immunoassays with coefficients of variation comparable with radioimmunoassay have been designed, values of under 5% being obtainable within the working range of the assay. |
| Databáze: | OpenAIRE |
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