Studies on drug-induced hemolysis: Effects of menadione and its water soluble preparations on the glutathione peroxidase of human erythrocytes
| Autor: | W. Barthelmai, M. Vetrella |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Drug
Erythrocytes Vitamin K media_common.quotation_subject In Vitro Techniques Hemolysis Hemoglobins chemistry.chemical_compound Menadione Drug Discovery medicine Humans Incubation Genetics (clinical) media_common chemistry.chemical_classification Glutathione peroxidase Cell Membrane Sodium General Medicine Glutathione medicine.disease Molecular biology Enzyme Peroxidases chemistry Biochemistry Molecular Medicine Hemoglobin Oxidation-Reduction |
| Zdroj: | Klinische Wochenschrift. 50:234-238 |
| ISSN: | 1432-1440 0023-2173 |
| DOI: | 10.1007/bf01486527 |
| Popis: | Incubation with MNS at a concentration of 2×10−5 M inhibits 51.7±15.6% of the GSH-px activity in hemolysates, while concentrations ranging between 5×10−5 and 1×10−4 M lead to 100% inhibition. 1×10−4 M MNS in hemoglobin free enzyme solution results in 70% inhibition. The incubation of hemolysates and Hb free GSH-px solution with menadione in concentrations ranging between 2 and 4×10−4 M results in 44 to 70% inhibition. The inhibition appears to be of the non-competitive type and not to be reversed by dialysis. Though MNP at a concentration of 2×10−2 M does not inhibit GSH-px both in hemolysates and Hb-free GSH-px preparations. Na2S2O5 at concentrations of 1×10−3 M produces 50% inhibition of GSH-px. The accumulation of H2O2 caused by the presence of the autoxidable menadione and MNS and by the inhibition of GSH-px might impair the GSH-mediated protective mechanism against oxidative damage in the erythrocytes. Consequently, further oxidation of hemoglobin and of SH groups of the red cell membrane could lead to hemolysis. |
| Databáze: | OpenAIRE |
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