Bacterial ghosts as a delivery system for zona pellucida-2 fertility control vaccines for brushtail possums (Trichosurus vulpecula)
| Autor: | Janine A. Duckworth, Phil E. Cowan, F.C. Molinia, Werner Lubitz, Jane Arrow, Petra Walcher, Xianlan Cui, Susie Scobie |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Recombinant Fusion Proteins
medicine.medical_treatment media_common.quotation_subject Fertility Andrology Drug Delivery Systems Immune system Adjuvants Immunologic Antigen Immunity Escherichia coli medicine Animals Vaccines Contraceptive Lymphocytes Pest Control Biological Zona pellucida Zona Pellucida Cell Proliferation media_common Immunity Cellular Bacteria General Veterinary General Immunology and Microbiology biology Reverse Transcriptase Polymerase Chain Reaction Artificial insemination Public Health Environmental and Occupational Health biology.organism_classification Infectious Diseases medicine.anatomical_structure Immunology biology.protein Molecular Medicine Brushtail possum Female Antibody Trichosurus New Zealand Plasmids |
| Zdroj: | Vaccine. 26:6832-6838 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2008.09.088 |
| Popis: | The introduced brushtail possum is a serious pest in New Zealand and there is much interest in the development of an immunocontraceptive vaccine for population control. Immunisation of female possums against recombinant possum zona pellucida protein-2 (ZP2) is known to reduce embryo production by 72-75% but successful development of fertility control will depend on a delivery system that is effective for field use. Bacterial ghost vaccine technology is a promising system to formulate a non-living vaccine for bait or aerosol delivery. The N-terminal (amino acid residues 41-316, ZP2N) and C-terminal (amino acid residues 308-636, ZP2C) regions of possum ZP2 were fused to maltose-binding protein and expressed in the periplasmic space of Escherichia coli NM522 bacterial ghosts. Female possums (n=20 per treatment group) were immunised with 20mg of either plain ghosts, ZP2N ghosts, or ZP2C ghosts in phosphate-buffered saline applied to the nostrils and eyes (nasal/conjunctival mucosa) at weeks 0, 2 and 4. Effects of immunisation on fertility were assessed following superovulation and artificial insemination. Both constructs evoked humoral (antibody) and cell-mediated immune responses in possums and significantly fewer eggs were fertilised in females immunised against ZP2C ghosts. Results in this study indicate that bacterial ghosts containing possum ZP antigens can reduce possum fertility when delivered by mucosal immunisation and offer a promising delivery system for fertility control of wild possum populations. |
| Databáze: | OpenAIRE |
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