Transient expression of S-100 by melanotropes of the rat pituitary intermediate lobe during development
| Autor: | Keith A. Gary, Scott A. Sands, Bibie M. Chronwall |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Pituitary gland Time Factors Molecular Sequence Data Melanophores Gestational Age Vimentin In situ hybridization Biology Glia maturation factor Rats Sprague-Dawley Embryonic and Fetal Development Developmental Neuroscience Internal medicine medicine Animals Connective Tissue Cells Base Sequence S100 Proteins Immunohistochemistry Rats Endocrinology medicine.anatomical_structure Animals Newborn Connective Tissue Pituitary Gland biology.protein Hepatic stellate cell Neuroglia Developmental Biology Neurotrophin |
| Zdroj: | International Journal of Developmental Neuroscience. 13:567-576 |
| ISSN: | 1873-474X 0736-5748 |
| DOI: | 10.1016/0736-5748(95)00037-h |
| Popis: | S-100, an acidic calcium-binding protein, is co-localized with vimentin in glial-like cells in the adult rat pituitary intermediate lobe. S-100 and melanotrope markers were not co-localized in the adult. During development, S-100 and vimentin were not co-localized but appeared in cells with different morphological characteristics. S-100 was co-localized with POMC mRNA and beta-endorphin during prenatal time and the first three postnatal weeks. This was demonstrated by double-label immunohistochemistry, using combinations of antisera against S-100, vimentin and beta-endorphin, and in situ hybridization histochemistry for POMC mRNA combined with immunohistochemistry for S-100. In the second and third weeks of postnatal development, S-100 was observed in fewer melanotropes and more frequently in stellate cells, which also expressed vimentin. Thus, S-100 appeared to be transiently expressed in melanotropes during prenatal and early postnatal development. S-100 serves as a neurotrophic and glial maturation factor in the CNS. Since S-100 expression in melanotropes coincides with the onset of dopaminergic innervation and morphological changes in glial-like cells of the lobe, it could have similar functions in the rat pituitary intermediate lobe. |
| Databáze: | OpenAIRE |
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