Chimeric envelope glycoproteins constructed between amphotropic and xenotropic murine leukemia retroviruses
| Autor: | Yawen Chiang, Tiffany Hofmann, W. French Anderson, Lei Han |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Recombinant Fusion Proteins
viruses Blotting Western Molecular Sequence Data EcoRI Cell Line Mice Chimera (genetics) Viral Envelope Proteins Viral envelope hemic and lymphatic diseases Genetics medicine Animals Polyproline helix chemistry.chemical_classification Base Sequence biology 3T3 Cells Cell Biology General Medicine medicine.disease Virology Molecular biology Amino acid Leukemia Virus Murine Leukemia chemistry DNA Viral biology.protein Artifacts Glycoprotein |
| Zdroj: | Somatic Cell and Molecular Genetics. 21:205-214 |
| ISSN: | 1572-9931 0740-7750 |
| Popis: | A set of chimeric envelope proteins between amphotropic and xenotropic murine leukemia retroviruses (MuLV), two closely related members in the MuLV family, were constructed. The purpose was to examine the regions that could be successfully exchanged between these two similar viral envelope proteins. The data indicate that fully active chimeras can be built when the junction is either at the EcoRI site (amino acid 169) 42 amino acids N-terminal to the polyproline hinge of gp70 (named CH4) or at the ScaI site (aa 593) in the membrane spanning portion of p15E (CH1). However, a chimera at the AflII site (aa 125, CH5) and two in the C-terminal end of gp70 (aa 418, CH2; aa 326, CH3) were inactive. These results, taken together with other data from our laboratory and others, suggest that the entire gp70/p15E structure is sensitive to alterations and that even envelope proteins that are very similar have only a limited ability to exchange sequences. |
| Databáze: | OpenAIRE |
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