Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba
| Autor: | Pierre Marquet, Tristan Colomb, Benjamin Rappaz, Edward A. D. Mitchell, Nicolas Pavillon, Christian Depeursinge, Florian Charrière, Thierry J. Heger |
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| Předmět: |
Materials science
Optical diffraction Holography Magnification Imaging systems : Tomography law.invention Optics Optical microscope law Holography : Computer holography Imaging systems : Three-dimensional image acquisition Amoeba (mathematics) Animals Tomography Optical Amoeba [MVD] Microscopy business.industry Resolution (electron density) Signal Processing Computer-Assisted Medical optics and biotechnology : Three-dimensional microscopy Image Enhancement Atomic and Molecular Physics and Optics Refractometry Digital holographic microscopy Tomography business Refractive index |
| Popis: | This paper presents an optical diffraction tomography technique based on digital holographic microscopy. Quantitative 2-dimensional phase images are acquired for regularly-spaced angular positions of the specimen covering a total angle of pi, allowing to built 3-dimensional quantitative refractive index distributions by an inverse Radon transform. A 20x magnification allows a resolution better than 3 microm in all three dimensions, with accuracy better than 0.01 for the refractive index measurements. This technique is for the first time to our knowledge applied to living specimen (testate amoeba, Protista). Morphometric measurements are extracted from the tomographic reconstructions, showing that the commonly used method for testate amoeba biovolume evaluation leads to systematic under evaluations by about 50%. |
| Databáze: | OpenAIRE |
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