Transcriptional response of Silicibacter pomeroyi DSS-3 to dimethylsulfoniopropionate (DMSP)
| Autor: | Helmut Bürgmann, Shulei Sun, Erinn C. Howard, Wenying Ye, Feng Sun, Mary Ann Moran, Sarah Napierala |
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| Rok vydání: | 2007 |
| Předmět: |
Quality Control
Transcription Genetic Sulfonium Compounds Biology Dimethylsulfoniopropionate Microbiology chemistry.chemical_compound Transcription (biology) Botany Rhodobacteraceae Gene Silicibacter pomeroyi Ecology Evolution Behavior and Systematics Acetic Acid Oligonucleotide Array Sequence Analysis chemistry.chemical_classification Gene Expression Profiling Alphaproteobacteria Biological Transport Gene Expression Regulation Bacterial Metabolism Roseobacter biology.organism_classification Amino acid chemistry Biochemistry Sulfur |
| Zdroj: | Environmental Microbiology. 9:2742-2755 |
| ISSN: | 1462-2920 1462-2912 |
| DOI: | 10.1111/j.1462-2920.2007.01386.x |
| Popis: | Summary Dimethylsufoniopropionate (DMSP) is an abundant organic sulfur compound in the ocean and an important substrate for marine bacterioplankton. The Roseobacter clade of marine alphaproteobacteria, including Silicibacter pomeroyi strain DSS-3, are known to be involved in DMSP degradation in situ. The fate of DMSP has important implications for the global sulfur cycle, but the genes involved in this process and their regulation are largely unknown. S. pomeroyi is capable of performing two major pathways of DMSP degradation, making it an important model organism. Based on the full genome sequence of this strain we designed an oligonucleotide-based microarray for the detection of transcripts of nearly all genes. The array was used to study the transcriptional response of S. pomeroyi cultures to additions of DMSP compared to the non-sulfur compound acetate in a time series experiment. We identified a number of upregulated genes that could be assigned to potential roles in the metabolism of DMSP. DMSP also affected the transcription of genes for transport and metabolism of peptides, amino acids and polyamines. DMSP concentration may thus also play a role as a chemical signal, indicating phytoplankton abundance and eliciting a regulatory response aimed at making maximum use of available nutrients. |
| Databáze: | OpenAIRE |
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