Endometrial Cancer Spheres Show Cancer Stem Cells Phenotype and Preference for Oxidative Metabolism
| Autor: | T.J. Gonçalves, Daniela Sarmento-Santos, João Casalta-Lopes, Catarina Mamede, Ana M. Abrantes, Tânia Costa, Nuno Almeida, Carlos Augusto Fernandes de Oliveira, Mafalda Laranjo, Maria João Carvalho, Rui de Albuquerque Carvalho, Artur Paiva, João Crispim Encarnação, Rui Caetano Oliveira, Beatriz Serambeque, Filomena Botelho |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Neoplasias do Endométrio Mice Nude Estrogen receptor Aldehyde dehydrogenase Apoptosis medicine.disease_cause Pathology and Forensic Medicine Mice 03 medical and health sciences 0302 clinical medicine Cancer stem cell Progesterone receptor Biomarkers Tumor Tumor Cells Cultured medicine Animals Humans AC133 Antigen Receptor beta Catenin Cell Proliferation Mice Inbred BALB C biology Chemistry Endometrial cancer CD44 General Medicine medicine.disease Xenograft Model Antitumor Assays Stress Oxidativo Endometrial Neoplasms Oxidative Stress Glucose Hyaluronan Receptors Phenotype 030104 developmental biology Oncology 030220 oncology & carcinogenesis Células Estaminais Neoplastic Stem Cells Cancer research biology.protein Female Carcinogenesis |
| Zdroj: | Pathology & Oncology Research. 25:1163-1174 |
| ISSN: | 1532-2807 1219-4956 |
| DOI: | 10.1007/s12253-018-0535-0 |
| Popis: | This study aimed to characterize endometrial cancer regarding cancer stem cells (CSC) markers, regulatory and differentiation pathways, tumorigenicity and glucose metabolism. Endometrial cancer cell line ECC1 was submitted to sphere forming protocols. The first spheres generation (ES1) was cultured in adherent conditions (G1). This procedure was repeated and was obtained generations of spheres (ES1, ES2 and ES3) and spheres-derived cells in adherent conditions (G1, G2 and G3). Populations were characterized regarding CD133, CD24, CD44, aldehyde dehydrogenase (ALDH), hormonal receptors, HER2, P53 and β-catenin, fluorine-18 fluorodeoxyglucose ([18F]FDG) uptake and metabolism by NMR spectroscopy. An heterotopic model evaluated differential tumor growth. The spheres self-renewal was higher in ES3. The putative CSC markers CD133, CD44 and ALDH expression were higher in spheres. The expression of estrogen receptor (ER)α and P53 decreased in spheres, ERβ and progesterone receptor had no significant changes and β-catenin showed a tendency to increase. There was a higher 18F-FDG uptake in spheres, which also showed a lower lactate production and an oxidative cytosol status. The tumorigenesis in vivo showed an earlier growth of tumours derived from ES3. Endometrial spheres presented self-renewal and differentiation capacity, expressed CSC markers and an undifferentiated phenotype, showing preference for oxidative metabolism. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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