Identification of Diverse Antimicrobial Resistance Determinants Carried on Bacterial, Plasmid, or Viral Metagenomes from an Activated Sludge Microbial Assemblage▿
| Autor: | Erin J. Consuegra, Kavita S. Kakirde, Larissa C. Parsley, Andrew M. Land, Willie F. Harper, Mark R. Liles |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Microbial Sensitivity Tests Biology medicine.disease_cause Applied Microbiology and Biotechnology DNA sequencing Microbiology Microbial Ecology Antibiotic resistance Plasmid Kanamycin Drug Resistance Bacterial medicine Escherichia coli Gene Library Genetics Ecology Sewage Circular bacterial chromosome Chloramphenicol Sequence Analysis DNA Anti-Bacterial Agents Metagenomics DNA Viral Metagenome Ampicillin Food Science Biotechnology medicine.drug Plasmids |
| Popis: | Using both sequence- and function-based metagenomic approaches, multiple antibiotic resistance determinants were identified within metagenomic libraries constructed from DNA extracted from bacterial chromosomes, plasmids, or viruses within an activated sludge microbial assemblage. Metagenomic clones and a plasmid that in Escherichia coli expressed resistance to chloramphenicol, ampicillin, or kanamycin were isolated, with many cloned DNA sequences lacking any significant homology to known antibiotic resistance determinants. |
| Databáze: | OpenAIRE |
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