The contribution of cytosolic group IVA and calcium-independent group VIA phospholipase A2s to adrenic acid mobilization in murine macrophages
| Autor: | Victoria Magrioti, Julio M. Rubio, Jesús Balsinde, María A. Balboa, Alvaro Garrido, George Kokotos, Patricia Monge |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Junta de Castilla y León, Instituto de Salud Carlos III |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
lcsh:QR1-502 Phospholipid Lipid signaling Inflammation Phospholipase Biochemistry lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Phospholipase A2 Adrenic acid medicine Macrófagos Molecular Biology Ácido araquidónico Innate immune system biology Ácido adrénico phospholipase A2 Mass spectrometry Macrophages Cytosol 030104 developmental biology chemistry Arachidonic acid Monocytes/macrophages 030220 oncology & carcinogenesis biology.protein Espectrometría de masas medicine.symptom |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Biomolecules Volume 10 Issue 4 UVaDOC. Repositorio Documental de la Universidad de Valladolid Biomolecules, Vol 10, Iss 542, p 542 (2020) |
| Popis: | This article belongs to the Special Issue Phospholipases: From Structure to Biological Function. Adrenic acid (AA), the 2-carbon elongation product of arachidonic acid, is present at significant levels in membrane phospholipids of mouse peritoneal macrophages. Despite its abundance and structural similarity to arachidonic acid, very little is known about the molecular mechanisms governing adrenic acid mobilization in cells of the innate immune system. This contrasts with the wide availability of data on arachidonic acid mobilization. In this work, we used mass-spectrometry-based lipidomic procedures to define the profiles of macrophage phospholipids that contain adrenic acid and their behavior during receptor activation. We identified the phospholipid sources from which adrenic acid is mobilized, and compared the data with arachidonic acid mobilization. Taking advantage of the use of selective inhibitors, we also showed that cytosolic group IVA phospholipase A2 is involved in the release of both adrenic and arachidonic acids. Importantly, calcium independent group VIA phospholipase A2 spared arachidonate-containing phospholipids and hydrolyzed only those that contain adrenic acid. These results identify separate mechanisms for regulating the utilization of adrenic and arachidonic acids, and suggest that the two fatty acids may serve non-redundant functions in cells. This research was funded by the Spanish Ministry of Economy, Industry, and Competitiveness, grant number SAF2016-80883-R. P.M. and A.G. were supported by predoctoral fellowships from the Regional Government of Castile and Leon. CIBERDEM is an initiative of Instituto de Salud Carlos III. |
| Databáze: | OpenAIRE |
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