GABA uptake by purified avian Müller glia cells in culture
| Autor: | Edna N. Yamasaki, Maria Christina Fialho de Mello, Ricardo Augusto de Melo Reis, Regina Célia Cussa Kubrusly, Clarissa S. Schitine, Fernando G. de Mello |
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| Rok vydání: | 2007 |
| Předmět: |
Nipecotic Acids
Stimulation Chick Embryo Biology Tritium Toxicology Inhibitory postsynaptic potential Retina GABA Antagonists chemistry.chemical_compound Chlorides Oximes medicine Animals Cells Cultured gamma-Aminobutyric Acid Neurons Analysis of Variance Dose-Response Relationship Drug General Neuroscience Sodium Temperature Glutamate receptor Biological Transport Transporter Coculture Techniques Cell biology medicine.anatomical_structure nervous system Biochemistry chemistry Inner nuclear layer sense organs Veratridine Neuroglia Muller glia |
| Zdroj: | Neurotoxicity Research. 12:145-153 |
| ISSN: | 1476-3524 1029-8428 |
| Popis: | GABA is the main inhibitory aminoacid transmitter present in neurons and glial cells. Its uptake is carried out by specific high-affinity Na(+)/Cl (-) dependent transporters (GATs). It has been reported in the past that, in the avian retina, [(3)H]GABA appears to be exclusively accumulated by horizontal and amacrine cells in the inner nuclear layer, and also by ganglion cells. Purified chick Müller glia cultures were able to take up [(3)H]GABA in a Na(+) and Cl(+) dependent way. Increasing GABA concentration increases GABA uptake by these cells, reaching half-maximal transport efficiency (EC50) around 0.3 mM. [(3)H]GABA uptake by Müller glia neuronal-free cultures was not mediated by neuronal transporters since it was not blocked by NNC-711, but was inhibited by beta-alanine, a specific glial transporter inhibitor. Chick Müller glia in culture express both GAT-1 and GAT-3 GABA transporters. Although mixed neuron-glial dense cultures released GABA upon glutamate, high K[(+) or veratridine stimulation, Müller glial cells did not release [(3)H]GABA upon treatment with these agents, suggesting that different from neurons, transporter mediated GABA release is not a common mechanism operating in these cells. The data also suggest that Müller cells take up GABA unidirectionally, which may constitute an important mechanism of inactivating GABA activity mediated by neurons. |
| Databáze: | OpenAIRE |
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