Activated platelet aggregates in thrombotic thromboctyopenic purpura: decrease with plasma infusions and normalization in remission
| Autor: | Yeon S. Ahn, Wenche Jy, Peter A. Valant, Robert C. Duncan, Wei Wei Mao, Lawrence L. Horstman, Luciano Kolodny |
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| Rok vydání: | 1996 |
| Předmět: |
Hemolytic anemia
medicine.medical_specialty Platelet Aggregation medicine.medical_treatment education Thrombotic thrombocytopenic purpura Gastroenterology Flow cytometry hemic and lymphatic diseases Internal medicine mental disorders Blood plasma medicine Humans Platelet Platelet activation Whole blood medicine.diagnostic_test Plasma Exchange Purpura Thrombotic Thrombocytopenic business.industry Hematology Plasmapheresis medicine.disease Flow Cytometry Platelet Activation Immunology business psychological phenomena and processes |
| Zdroj: | British journal of haematology. 95(2) |
| ISSN: | 0007-1048 |
| Popis: | Circulating activated platelet aggregates (aPA) were assayed by flow cytometry employing mAb alpha-CD62p in eight patients with thrombotic thrombocytopenic purpura (TTP). Elevation of aPA was observed in all patients in active stages of TTP; aPA normalized in remission. Plasma infusions with plasmapheresis decreased aPA in responding patients. The rise and fall of aPA preceded relapses and improvements, respectively. These changes were seen prior to the traditional indicators, LDH, haematocrit, and platelet count. Incubation of plasma from TTP patients with normal whole blood induced formation of aPA; this effect was significantly greater than that of plasmas from ITP patient controls (P < 0.01), suggesting the presence of an aPA-promoting factor in TTP plasma. Parallel experiments using a platelet aggregometer failed to detect effect of TTP plasma on normal blood. In summary, aPA appear to be a marker of disease activity, rising with relapse, falling with plasma therapy, and normalizing in remission. The flow cytometric assay of aPA is more sensitive than aggregometry in detecting the putative aPA-promoting factor in TTP. |
| Databáze: | OpenAIRE |
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