Evaluation of Antioxidant, Antibacterial and Enhancement of Antibiotic Action by Punica granatum Leaves Crude Extract and Enriched Fraction against Multidrug‐Resistant Bacteria
| Autor: | Wliana Alves Viturino da Silva, Thamara Figueiredo Procópio, Maiara Celine de Moura, Janaina Carla Barbosa Machado, Magda Rhayanny Assunção Ferreira, Luiz Alberto Lira Soares, Thiago Henrique Napoleão, Ewelyn Cintya Felipe dos Santos, Liliane Bezerra de Lima |
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| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
medicine.drug_class DPPH medicine.medical_treatment Antibiotics Ethyl acetate Bioengineering Microbial Sensitivity Tests Biochemistry Antioxidants chemistry.chemical_compound Picrates Drug Resistance Multiple Bacterial Escherichia coli medicine Benzothiazoles Food science Phenols Molecular Biology Lythraceae Plant Extracts Biphenyl Compounds General Chemistry General Medicine Antimicrobial Thin-layer chromatography Anti-Bacterial Agents Plant Leaves Klebsiella pneumoniae chemistry Pseudomonas aeruginosa Molecular Medicine Sulfonic Acids Antibacterial activity |
| Zdroj: | Chemistry & Biodiversity. 18 |
| ISSN: | 1612-1880 1612-1872 |
| DOI: | 10.1002/cbdv.202100538 |
| Popis: | The aim of this study was to evaluate the phytochemical composition, antioxidant, and antimicrobial potential of crude extract and fractions of Punica granatum leaves. The extract was produced by turbo extraction, after which hexanic, ethyl acetate, and aqueous fractions were obtained by partitioning. The chemical analyses were performed by thin layer chromatography and high-performance liquid chromatography, and the antioxidant activities were assayed by DPPH. and ABTS.+ . Minimal inhibitory and bactericidal concentrations (MIC/MBC) were applied to twenty-two bacteria. Most strains susceptible to extract/fractions and resistant to antibiotics were selected, and ampicillin, azithromycin, ciprofloxacin, and gentamicin were associated with the ethyl acetate fraction (EAF) against multidrug-resistant strains in modulatory and checkboard models. The data from chromatographic analyses showed flavonoids and tannins in the extract, as well as the enrichment of EAF in phenols, mainly flavonoids. The flavonoids were connected to the electron transfer activity demonstrated in the DPPH. and ABTS.+ assays. Gram-positive strains are more susceptible to EAF. The subinhibitory concentrations of P. granatum enhanced the antimicrobial activity of the agents and reduced the EAF individual MIC, and the combination of EAF and antibiotics demonstrated a synergistic effect. These results present a promising approach for developing a therapy in which antioxidant extracts and fractions can be used in combination with antibiotics. |
| Databáze: | OpenAIRE |
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